Table 1 Reduction of lin-41 function reduces oocyte quality and increases meiotic chromosome nondisjunction
GenotypeTemperature% FertileaBrood sizeb% Male
WTc25°100 (n = 258)201.5 ± 61.8 (n = 30)0.1 (n = 6044)
lin-41(tn1487 ts)15°100 (n = 224)103.8 ± 37.8d (n = 64)0.3 (n = 6642)
lin-41(tn1487 ts)22°83.7 (n = 86)3.9 ± 3.8e (n = 86)2.4 (n = 338)
lin-41(tn1487 ts)c25°0.3 (n = 310)0 ± 0.1 (n = 168)ND
lin-41(tn1487 tstn1515)c25°98.1 (n = 472)66 ± 36 (n = 31)1.6 (n = 435)
lin-41(tn1487 tstn1516)c25°99.7 (n = 324)108 ± 36 (n = 25)2.5 (n = 1586)
lin-41(tn1487 tstn1536)c25°33.9 (n = 230)5.4 ± 6.3f (n = 83)5.1 (n = 455)
lin-41(tn1487 tstn1539)c25°86.2 (n = 232)14.0 ± 10.9g (n = 82)5.7 (n = 1152)
  • a Fertile animals produced at least one viable offspring.

  • b Number of progeny hatching per adult.

  • c Newly fertilized embryos were collected at 15° and shifted to 25°. The fertility, brood size, incidence of male progeny, and embryonic lethality were measured by examination of the F1 progeny.

  • d lin-41(tn1487 ts) adults lay 139.7 eggs per adult (n = 32), with 75.9% hatching at 15°.

  • e lin-41(tn1487 ts) adults lay 9.8 eggs per adult (n = 29), with 39.1% hatching at 22°.

  • f lin-41(tn1487 tstn1536) adults lay 29.1 eggs per adult (n = 39), with 20.1% hatching at 25°.

  • g lin-41(tn1487 tstn1539) adults lay 57.3 eggs per adult (n = 40), with 27.3% hatching at 25°.