Table 1 Proteins that copurify with OMA-1 and either interact with OMA-1 or regulate mRNA translation, cytoplasmic polyadenylation, or deadenylation
ProteinCoverage (%)aKnown physical interaction or phenotypic similarity
 PQN-599Interacts with OMA-1 (Figure 7)
mRNA translation
 GLD-124Interacts with OMA-1 (Figure 7)
 LIN-4119Represses OMA targets (Figure 8)
 MEX-347Interacts with OMA-1 (Figure 7)
 OMA-221Interacts with OMA-1 (Figure 7)
 MEX-126None
 PUF-510Enhances oma-2(te51) and represses glp-1 translation in oocytes (this work; Lublin and Evans 2007)
 SPN-46Interacts with OMA-1 (Figure 7)
 IFG-110None
 IFE-314Interacts with IFET-1 (Li et al. 2009)
 IFET-16Interacts with OMA-1 and represses OMA targets in oocytes (Li et al. 2009; Guven-Ozkan et al. 2010; Oldenbroek et al. 2013)
 POS-121Interacts with SPN-4 (Ogura et al. 2003) and is expressed in oocytes (T. Guven-Ozkan and R. Lin, unpublished results)b
Cytoplasmic polyadenylation
 GLD-321None
 GLD-216None
Deadenylation
 LET-7114None
 CCF-118None
 NTL-911None
  • a Peptide coverage in a single gel slice assessed by mass spectrometry in an OMA-1 purification after RNase treatment. OMA-1 was purified from a fog-1(ts) female lysate.

  • b POS-1 expression was detected in oocytes by antibody staining (using antibodies described in Tabara et al. 1999), albeit at a level lower than in embryos (T. Guven-Ozkan and R. Lin, unpublished results).