Table 2 Regulation of HR proteins by post-translational modifications
TargetPTMModifier(s)FunctionReferences
Sae2S267PCdc28Promotes resectionAylon et al. (2004); Huertas et al. (2008); Ira et al. (2004); Zierhut and Diffley (2008)
PTel1/Mec1Activation through solubilizationBaroni et al. (2004); Fu et al. (2014)
Ac?DegradationRobert et al. (2011)
Dna2T4P, S17P, S237PCdc28Promotes long-range resectionChen et al. (2011)
S17PCdc28Nuclear localizationKosugi et al. (2009)
Rad55S2P, S8P, S14PMec1?Bashkirov et al. (2000); Herzberg et al. (2006)
Rad51S192PMec1ATPase regulationFlott et al. (2011)
Srs2PCDC28Inhibits displacement of Rad51Saponaro et al. (2010)
K1081S, K1089S, K1142S?Promotes displacement of Rad51Saponaro et al. (2010)
Rad52K43S, K44S, K253SSiz2Inhibits DNA binding and annealing of ssDNAAltmannova et al. (2010); Sacher et al. (2006)
UbSlx5–Slx8?(Ii et al. (2007)
Slx4T113PMec1/Tel1Activation of Rad1-Rad10 nucleaseToh et al. (2010)
PMec1/Tel1Interaction with Dpb11Ohouo et al. (2010)
Mms4PCDC28, Cdc5Activation in M phaseLoog and Morgan (2005); Matos et al. (2011)
Yen1PCDC28, Cdc5Inhibition outside of anaphaseLoog and Morgan (2005); Matos et al. (2011)
S655P, S679PCDC28Nuclear localizationEissler et al. (2014); Kosugi et al. (2009)
Rad1K32SSiz1, Siz2Inhibition of DNA bindingSarangi et al. (2014)
PCNAK127S, K164S?Binding of Srs2Hoege et al. (2002); Papouli et al. (2005)
K164UbRad6–Rad18Promotes translesion synthesisHoege et al. (2002)
K164UbnRad5–Ubc13–Mms2Promotes error-free lesion bypass by template switchingBlastyak et al. (2007); Hoege et al. (2002)
Rtt107PMec1 (Slx4)Replication restartRoberts et al. (2006)
Mcm2–7S164P, S170PMec1 (Mrc1)Replication restartRandell et al. (2010); Stead et al. (2012)
  • P, phosphorylation. Ac, acetylation. S, sumoylation. Ub, ubiquitylation. Parentheses indicate partial dependency. ?, unknown.