Table 1 Bacterial strains and plasmids
Strain nos.Genotypes/descriptionReference or source
E. coli K12
 TR7177ara thiA ∆(lac-proB)XIII/F′128 proAB+ ∆(lacI-Z), same as FC29Cairns and Foster (1991)
 TR7178ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω), same as FC40Cairns and Foster (1991)
 TT26180F ara thiA rifR ∆(lac-proB)XIII, same as FC36Cairns and Foster (1991)
 TT26323ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) yebB11::Tn10dTcThis study
 TT26324ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) yagU211::Tn10dTcThis study
 TT26325ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) ychA11::Tn10dTcThis study
 TT26326ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) tnpR11::Tn10dTcThis study
 TT26327ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) lacA4659::Tn10dTcThis study
 TT26328ara thiA rifR ∆(lac-proB)XIII btuR482::Tn10dTc/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω)This study
 TT26900FC40/pSMP71 (pOU71::tetRA-gfp-cat)This study
 TT26905ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) zzf-1831(mhpC)::Tn10dTc-gfp-cat yebB11::Tn10dTcThis study
 TT26911FC40/pSMP73 (pGB2:: tetRA-gfp-cat)This study
 TT26916ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) zzf-1831(mhpC)::Tn10dTc-gfp-catLaboratory collection
 TT26932aara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) yebB11::Tn10dTcThis study
 TT26933aara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) lacA4659::Tn10dTcThis study
 TT26935ara thiA rifR ∆(lac-proB)XIII/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω) lacA4659::Tn10dTc yebB11::Tn10dTcThis study
S. typhimurium LT2
 TT18784hisG10175::Tn10dTc hisC9955::MudJLaboratory collection
 TT25387leuD21 proAB670::SpecLaboratory collection
 TT26310leuD21 proAB670::cam recA651::rif/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω)Laboratory collection
 TT26311leuD21 proAB670::cam recA651::rif/F′128 proAB+ lacIq lacI33(fs) lacIZ(Ω)/pNK2881This study
Plasmid
 pNK2881Tn10 transposase under tac promoter, ApRWay et al. (1984)
 pGB2pSC101 origin, SpRChurchward et al. (1984)
 pOU71Temperature-dependent R1 origin, ApRLarsen et al. (1984)
 pSMP71pOU71::tetRA-gfp-cat, ApR, TcR, CmRThis study
 pSMP73pGB2::tetRA-gfp-cat, SpR, TcR, CmRThis study
  • a Reconstructed duplicates.