Table 1 Properties of strains used in this study
RpoS/RpoD ratio (m ± SD)a
StrainRelevant genotype (primer set used for construction)Exponential bacteriabStationary phase bacteriabSource
MC4100F- araD139 Δ(argF-lac)U169 rspL150 deoCl relA1 thiA ptsF25 flb5301 rbsR0.00 ± 0.000.40 ± 0.06Ferenci et al. (2009)
BW2952malG::λplacMu55 ϕ(malG::lacZ)rssB::IS10.76 ± 0.041.00 ± 0.00Ferenci et al. (2009)
BW3709BW2952 rpoS::Tn100.00 ± 0.000.00 ± 0.00Notley-Mcrobb et al. (2002)
BW5205BW2952 rpoSleader::bla (rpoSF and rpoSR1)0.30 ± 0.070.31 ± 0.02Maharjan et al. (2013)
BW5206BW2952 rpoSleader::bla (rpoSF and rpoSR2)0.01 ± 0.010.00 ± 0.01Maharjan et al. (2013)
BW5207BW2952 rpoSleader::bla (rpoSF and rpoSR3)0.13 ± 0.090.16 ± 0.10Maharjan et al. (2013)
BW5208BW2952 rpoSleader::bla (rpoSF and rpoSR4)0.86 ± 0.040.72 ± 0.13Maharjan et al. (2013)
BW5213BW2952 rpoSleader::bla (rpoSF and rpoSR5)0.28 ± 0.030.22 ± 0.05Maharjan et al. (2013)
BW5214BW2952 rpoSleader::bla (rpoSF and rpoSR6)0.28 ± 0.010.27 ± 0.06Maharjan et al. (2013)
BW5219BW2952 rpoSleader::bla (rpoSF and rpoSR7)0.26 ± 0.030.22 ± 0.01Maharjan et al. (2013)
BW5220BW2952 rpoSleader::bla (rpoSF and rpoSR8)0.02 ± 0.000.03 ± 0.01Maharjan et al. (2013)
BW5222BW2952 rpoSleader::bla (rpoSF and rpoSR10)0.31 ± 0.000.38 ± 0.03Maharjan et al. (2013)
  • a RpoS/RpoD ratio data for the two culture conditions are from graphs in Maharjan et al. (2013) based on Western blot analysis of RpoS and RpoD detected using anti-RpoS and anti-RpoD antibodies. The mean (m) and standard deviation (SD) were from at least three independent experiments.

  • b The growth medium used was Luria-Bertani broth.