Table 1 inx-8(tn1474) inx-9(ok1502) mutants exhibit a severe germline proliferation defect
Average no. of germ cells per animala
GenotypeL2–L3L3–L4L4–YAAdult
WT76 ± 21 (n = 12)186 ± 37 (n = 9)∼1000b∼2000b
inx-8 inx-95.3 ± 3.3 (n = 39)7.4 ± 5.9 (n = 11)3.3 ± 1.6 (n = 24)2.5 ± 2.3 (n = 13)
  • a The number of germ cells was determined by anti-GFP staining of PGL-1::GFP-positive germ cells in sem-3(n1655) inx-8(tn1474) inx-9(ok1502); neIs5[pgl-1::gfp] animal whole mounts from mixed-stage cultures. Larval, young adult (YA), and adult stages were approximated by DAPI staining.

  • b Germ cell numbers are from Francis et al. (1995b) and are presented for comparison.