Table 3 Some resources available to yeast geneticists
NameWebsite/company/referenceResource
Saccharomyces Genome Databasehttp://www.yeastgenome.org/ (Cherry et al. 2012)Gene annotations, data from high-throughput screens, and publications with links to other databases and sequence information
Yeast deletion projecthttp://www-sequence.stanford.edu/group/yeast_deletion_project/deletions3.html (Brachmann et al. 1998; Winzeler et al. 1999)Yeast community cooperative project to systematically delete each nonessential ORF
Yeast deletion collectionThermo ScientificYeast strains with a deletion of a specified ORF can be purchased as individual strains or as a collection
Designer deletion strainsThermo Scientific (Brachmann et al. 1998)Set of strains developed for efficient one-step gene replacement using common yeast markers
pRS plasmid collectionAmerican Type Culture Collection (Sikorski and Hieter 1989) (Christianson et al. 1992)Low-copy (CEN), high-copy (2-μm), and integrating plasmids containing common yeast markers
Yeast GFP-tagged ORFsLife Technologies (Huh et al. 2003)Yeast strains containing individual ORFs fused to GFP at the C terminus
Yeast TAP-tagged ORFsThermo scientific (Ghaemmaghami et al. 2003)Yeast strains containing individual ORFs fused to a TAP tag at the C terminus
Yeast GFP Fusion Localization Databasehttp://yeastgfp.yeastgenome.org/Database of ORF–GFP fusions with images searchable by ORF name or cellular location
S. cerevisiae strain sequence alignmenthttp://www.yeastgenome.org/cgi-bin/FUNGI/alignment.plBioinformatics tool for aligning sequences within S. cerevisiae strains
Yeast sequence strain alignmenthttp://www.yeastgenome.org/cgi-bin/FUNGI/showAlignBioinformatics tool for aligning sequences among yeast species
DNA Replication Origin Databasehttp://cerevisiae.oridb.org/ (Nieduszynski et al. 2007; Siow et al. 2012)Bioinformatics tool for searching location of ARS across the yeast genome