Table 3 Primers used in this study

	Primer name	Primer sequence	Imbedded RE
1	GAD·Fob1·5′	`5′-ATGCTA GGATCCAC⁴ACGAAACCGCGTTACAATG²²`	BamHI
2	GAD·Fob1·3′	`5′-ATGCTA GTCGAC¹⁷⁰¹TTACAATTCCATTGATGTG¹⁶⁸³`	SalI
3	Fob1·Dom2·3′	`5′-ATGCTA AGATCT A ¹²⁵⁸CATTAGCAAGGGCAAAAG¹²⁴¹`	BglII
4	Fob1·Dom3·5′	`5′-ATGCTA GGATCC¹²⁵⁹AAGCGGATAATAGCTGTAAC¹²⁷⁸`	BamHI
5	Fob1·N-term·3′	`5′-ACGT GTCGAC⁸⁴⁹AATTGGAACCCTAGCAAATG⁸²⁹`	SalI
6	Fob1·C-term·5′	`5′-ACGT GGATCC⁸⁵⁰ACTTCGTAACATCAAGCATCTTA G⁸⁶⁸`	BamHI
7	E420V 3′	`5′-ACGTACGTG¹²⁹¹GAATTC CATTATTGTTACAGCTATTATCCGCAACgTTAGCAAGGG¹²⁴⁷`	EcoRI/AclI

The underlined sequences reflect restriction sites imbedded for cloning purposes. The nucleotide in bold in primer 7 was mutated to create the E420V mutation, which also created the new AclI restriction site. The superscript numbers within the primer sequences define where in the FOB1 DNA sequence the primers reside.
RE, restriction enzyme.