Table 2 Plasmids used in this study
PlasmidMarkersSource/reference
pVA3p53 2μ-TRP1 2 hyb·GAL4BDW. Neupert
pTD1SV40 large T-antigen 2μ-LEU2 2 hyb·GAL4ADW. Neupert
pGBT92μ-TRP1 2 hyb·GAL4BDW. Neupert
pGBT-APC52µ-TRP1 2 hyb·GAL4BDThis study
YCp50CEN-URA3W. Neupert
pTH54YCp50-APC5∆3′69Harkness et al. 2002
YEplac195-FOB12μ-URA3T. Kobayashi
pGAD·C22μ-LEU2 2 hyb·GAL4ADE. Craig
pRIN12, 20FOB15-1263 2μ-LEU2 2 hyb·GAL4ADThis study
pCR4-TOPOBacterial PCR cloning vectorInvitrogen
topo-FOB1pCR4-TOPOThis study
pGAD-FOB12μ-LEU2 2 hyb·GAL4ADThis study
pGAD-FOB1D12μ-LEU2 2 hyb·GAL4ADThis study
topo-FOB1D2pCR4-TOPOThis study
pGAD-FOB1D22μ-LEU2 2 hyb·GAL4ADThis study
topo-FOB1D3pCR4-TOPOThis study
pGAD-FOB1D32μ-LEU2 2 hyb·GAL4ADThis study
pGAD-FOB1D1/22μ-LEU2 2 hyb·GAL4ADThis study
topo-FOB1N-termpCR4-TOPOThis study
pGAD-FOB1N-term2μ-LEU2 2 hyb·GAL4ADThis study
topo-FOB1C-termpCR4-TOPOThis study
pGAD-FOB1C-term2μ-LEU2 2 hyb·GAL4ADThis study
pBG1805GALpro.6X His·HA·Prot A 2µ-URA3W. Xiao
GALpro-FOB1-HAGALpro.6X His·HA·Prot A 2µ-URA3W. Xiao
GALpro-FOB1E420V-HAGALpro.6X His·HA·Prot A 2µ-URA3This study
GALpro-FOB1DB1-HAGALpro.6X His·HA·Prot A 2µ-URA3This study
GALpro-FOB1DB2-HAGALpro.6X His·HA·Prot A 2µ-URA3This study
GALpro-FOB1DB1/DB2-HAGALpro.6X His·HA·Prot A 2µ-URA3This study
pYEX-APC5-GSTURA3/AMP CuPThis study
pPAS-ADE2CEN-ADE2W. Xiao