Table 2 Number of transgenic F1 and mutant F2 progeny produced using Cas9/sgRNA directed against genomic loci
sgRNATransgenic F1
Method/targetConcentrationaPhsp-16.48::Cas9 concentrationaNo. P0 injectedNo. selectedWith mutant progeny
U6 × lin-5202020925
U6 × lin-5505020245
T7 × lin-5105020290
T7 × lin-515050201240
U6 × rol-12020401441
U6 × rol-15050201402
U6 × dpy-11505020202
U6 × unc-119505020412
  • a All concentrations are in nanograms per microliter. Injections with 20 ng/µl Cas9/sgRNA are supplemented with 35 ng/µl of PstI-digested λ DNA. All injections include 5 ng/µl of the Pmyo-3::mCherry marker to identify transgenic animals.