Table 1 Summary of RGEN-mediated mutagenesis performed in this study
Target geneInjected P0No. of analyzed F1No. of F1 with mutationsMutation frequency (%)No. of analyzed F2No. of F2 with mutationsMutation frequency (%)
dpy-3A2411/24 (4.2)
B2400
C2400
D3333/33 (9.1)a242424/24 (100)
E1600
Total12144/121 (3.3)
unc-1A3255/32 (16)
B2444/24 (17)
C1800
D2200
Total9699/96 (9.4)
  • a F1 mutant animals derived from the D P0 animal were labeled as D-1, D-2, and D-3. Although D-3 F1 itself was not examined because of its loss during the experiment, the F2 animals from D-3 F1 were subjected to sequence analysis. See the main text for details.