Table 1 Mutants generated by CRISPR-Cas
GeneAlleleMutationSequence
dpy-11Wild typeNoneagatccttgcAAGCTGGGCACCATGGAGCAtggctggaatttt
sy74023-bp deletionagatccttg−−−−−−−−−−−−−−−−−−−−−−−gctggaatttt
sy7417-bp deletionagatccttgcAAGCTGGGCACC−−−−−−−Atggctggaatttt
sy742>3-kb deletionND
sy7431031-bp deletion−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
sy745RearrangementND
sy746>3-kb deletionND
sy747>3-kb deletionND
sy748565-bp deletionagatccttgcAAGCTGGGCACCAT−−−−−−−−−−−−−−−−−
sy749>3-kb deletionND
sy750NDND
unc-4Wild typeNonetcatcaacagGTTATCGTCATCCGGTGACGtggattgttcccg
sy744>2-kb deletionND
  • Shown are a list and a description of the targeted mutants generated using CRISPR-Cas. Wild-type sequences are included for comparison. The 20-bp sequence cloned into the sgRNA targeting each gene is in uppercase and boldface type; note that in the wild type it is followed by an NGG “PAM”. Deleted nucleotides are indicated with dashes. The precise ends of especially large deletions were not determined. sy745 is believed to be a rearrangement: sequences near the target site are present by PCR and by high-throughput sequencing, but attempts to use PCR to amplify the target locus were unsuccessful. sy750 is believed to be an off-site mutation induced by homology-directed double-strand break repair or to be a deletion-duplication, as a wild-type copy of the locus targeted for Cas9 cleavage can be amplified from this mutant. ND, not determined.