Table 2 Mutation frequencies induced by Cas9/gRNA at six genomic loci in Drosophila
Target LocusLocationChromatinEmbryos injectedLarvae hatchedNumber of adult F0 (crossed)Number of adult F0 (fertile)Indel-yielding F0/ total F0 examined (n/n)Fraction of mutant F1 (detected/examined)Possible phenotype of F0
FemaleMaleFemaleMale
CG142513REu.373111201780a100% (24/24)98.9%Male sterile
(K81)(87/88)
CG3708XEu.3501604018351781.8% (36/44)34.8%
(54/155)
CG96523REu.380165374336272.1%
(2/94)
CG17629YHet.4201343729330a100% (29/29)Male sterile
(kl-3)
CG180282LHet.4451283938303318%
(light)(9/50)
CG174203LHet.8001140b0bEarly larva
(RpL15)Lethal
  • a All males are sterile.

  • b No adults, embryonic (or first instar) lethal.