Table 1 Summary of major public Drosophila and Caenorhabditis elegans RNAi reagent collections
RNAi collectionReagent typeAll reagentsTarget 1 gene, all isoformsTarget 1 gene, not all isoformsTarget multiple genesReagents with >5 OTEs (19 bp)Target pseudogeneNo gene target
DKFZdsRNA, cell based20,01615,948 (80%)816 (4%)1466 (7%)394 (2%)78 (0.4%)1708 (9%)
DRSC-Genome LibrarydsRNA, cell based24,03719,615 (82%)1664 (7%)979 (4%)552 (2%)84 (0.4%)1695 (7%)
DRSC-Followup LibrarydsRNA, cell based94488519 (90%)470 (5%)296 (3%)15 (0.2%)14 (0.2%)149 (2%)
NIGdsRNA, transgenic fly11,72510,328 (88%)532 (5%)436 (4%)416 (4%)2 (0.02%)427 (4%)
TRiP-LongHairPindsRNA, transgenic fly24832255 (91%)114 (5%)72 (3%)8 (0.3%)2 (0.1%)40 (2%)
TRiP-ShortHairPinshRNA, transgenic fly41323738 (90%)242 (6%)120 (3%)0 (0%)2 (0.1%)30 (1%)
VDRC-GD LibrarydsRNA, transgenic fly21,80818,607 (85%)962 (4%)1357 (6%)745 (3%)60 (0.3%)822 (4%)
VDRC-KK LibrarydsRNA, transgenic fly10,7489135 (85%)431 (4%)378 (4%)67 (1%)27 (0.3%)777 (7%)
Ahringer LibrarydsRNA, worm feeding16,25611,002 (68%)678 (4%)1733 (11%)1074 (7%)283 (2%)2843 (16%)
  • DKFZ, German Cancer Research Center; DRSC, Drosophila RNAi Screening Center; NIG, National Institute of Genetics (Japan); TRiP, Transgenic RNAi Project; VDRC, Vienna Drosophila RNAi Center.