Table 1 NHEJ mutagenesis at the yellow locus by Cas9/gRNA
Target locusParentsNo. of F0 (crossed)No. of F0 (fertile)Indel yielding F0/total fertile F0n/n, (%)Mosaic F0/ total F0 examined n/n, (%)Detected mutants/total F1 progeny n/n, (%)
y1Female22175/17 (29.4)26/352 (7.4)
Male383020/30 (66.7)24/30 (80%)60/475 (12.6)a
y2Female524124/41 (58.5)94/1111 (8.5)
Male292215/22 (68.2)10/28 (35.7%)30/418 (7.2)a
  • a The female progeny of F1 was counted as total progeny.