Table 2 sad-4Δ and sad-5Δ act as semidominant suppressors of MSUD
Experiment 1::act+ (F2-35)::bmlR (F2-36)asm-1Δ (F2-25) (%)rΔ (F2-29) (%)
 WT (P3-08)36.0 × 1030.6 × 1032.70.8
 sad-4Δ (P11-43)173.2 × 10355.9 × 10361.652.4
 sad-1Δ (P3-25)285.0 × 103107.4 × 10381.099.0
(spores)(spores)(black)(football)
Experiment 2::act+ (F2-37)::bmlR (F2-38)asm-1Δ (F3-24) (%)rΔ (F2-27) (%)
 WT (P3-07)28.8 × 1030.6 × 1030.70.0
 sad-5Δ (P17-57)118.9 × 10312.0 × 10315.90.4
 sad-1Δ (P8-18)342.9 × 103398.7 × 10383.498.7
(spores)(spores)(black)(football)
  • Each MSUD tester (::act+, ::bmlR, asm-1Δ, or rΔ) is designed to cause the unpairing of a reporter gene during meiosis. When MSUD is proficient (i.e., tester × WT), these unpairings lead to the reduced production of black American football (spindle)-shaped ascospores. When MSUD is suppressed, the phenotypes can be partially (e.g., tester × sad-4Δ/5Δ) or near-fully (e.g., tester × sad-1Δ) restored to normal, depending on the strength of the suppressor.