Table 1 Summary of the karyotype changes associated with the CNV reporter in the CFR clones.
HaploidsDiploids
WTmsh2ΔWTmsh2Δymrcty1-5Δ
CFR clones isolated2622292216
Positive CFR upon retest2421262110
Karyotype changes
 Chr5 segmental duplicationa225520
 Chr13/chr5 translocationb002083
 Chr7/chr5 translocation00005
 Chr5 isochromosomec00001
 Chr5 aneuploidyd00001
 No karyotype change2161110
CFR clones with CGH data9591010
  • a Longer than normal chr5 molecules harboring tandem duplications or triplications of a segment of chr5 containing the CNV reporter flanked by direct repetitive full-length Ty1 or solo δ LTR elements.

  • b CFR511, a clone derived from the ymrcty1-5Δ homozygous diploid (JAY510), had a complex rearrangement consisting of a deletion of the right arm of chr13 from YMRCTy1-4 to the right telomere, joined by a segment of chr5 containing a quadruplication of the segment containing the CNV reporter between YERCTy1-1 and YERCδ26, in addition to one copy of the terminal segment between YERCδ26 and TEL05R.

  • c CFR510, a clone derived from the ymrcty1-5Δ homozygous diploid (JAY510), had an isochromosome 5 formed by loss of the left arm from TEL05L to the Watson Ty1 element inserted at the ura3-52 allele, joined by the segment from right arm from YERCTy1-1 to TEL05R. This isochromosome was present in addition to the two parental size copies of chr5, both of which were retained in CFR510.

  • d CFR512, a clone derived from the ymrcty1-5Δ homozygous diploid (JAY510), had three copies of the parental size chr5, a simple aneuploidy of chr5.