Table 1  Phenotypic analysis of shRNA lines
LineGene nameVectorGal4 lineF1 phenotypeF2 phenotype
Mat genes
 HMS00930nanos (nos)VALIUM20mat-tub-Gal4No100%, nanos
 GL00407bicoid (bcd)VALIUM22mat-tub-Gal4No100%, bicoid
 GL01320bicoid (bcd)VALIUM22MTD-Gal4No100%, bicoid
 HMS00727dorsal (dl)VALIUM20mat-tub-Gal4No100%, dorsalized
 GL00610dorsal (dl)VALIUM22mat-tub-Gal4No100%, dorsalized
 GL00222torso (tor)VALIUM22mat-tub-Gal4No80%, weak torso
 HMS00021torso (tor)VALIUM20mat-tub-Gal4No100%, torso
Zyg genes
 HMS00595engrailed (en)VALIUM20mat-tub-Gal4NoNo
 HMS01312even skipped (eve)VALIUM20mat-tub-Gal4NoNo
 HMS01105giant (gt)VALIUM20mat-tub-Gal4NoNo
 GL01317giant (gt)VALIUM22MTD-Gal4NoNo
 GL01318giant (gt)VALIUM22MTD-Gal4NoNo
 GL01319giant (gt)VALIUM22mat-tub-Gal4NoNo
 HMS00492hedgehog (hh)VALIUM20mat-tub-Gal4NoNo
 HMS01216huckebein (hkb)VALIUM20mat-tub-Gal4NoNo
 HMS01184knirps (kni)VALIUM20mat-tub-Gal4NoNo
 HMS01106Kruppel (Kr)VALIUM20mat-tub-Gal4NoNo
 GL01322Kruppel (Kr)VALIUM22MTD-Gal4NoNo
 GL01323Kruppel (Kr)VALIUM22mat-tub-Gal4NoNo
 GL01324Kruppel (Kr)VALIUM22MTD-Gal4NoNo
 HMS01186runt (run)VALIUM20mat-tub-Gal4NoNo
 HMS01108sloppy paired 2 (slp2)VALIUM20mat-tub-Gal4NoNo
 HMS01313hairy (h)VALIUM20mat-tub-Gal4NoNo
 HMS01317twist (twi)VALIUM20mat-tub-Gal4NoNo
 HMS01215brother of odd with entrails limited (bowl)VALIUM22mat-tub-Gal4NoNo
 HMS01122crocodile (croc)VALIUM20mat-tub-Gal4NoNo
 HMS01150Dichaete (D)VALIUM20mat-tub-Gal4NoNo
 HMS01103forkhead (fkh)VALIUM20mat-tub-Gal4NoNo
 HMS01104fushi tarazu (ftz)VALIUM20mat-tub-Gal4NoNo
 HMS01552knirps like (knl)VALIUM20mat-tub-Gal4NoNo
 HMS01315odd-skipped (odd)VALIUM20mat-tub-Gal4NoNo
 HMS01314orthodenticle (otd)VALIUM20mat-tub-Gal4NoNo
 HMS01167schnurri (schn)VALIUM20mat-tub-Gal4NoNo
 HMS01107sloppy paired 1 (slp1)VALIUM20mat-tub-Gal4NoNo
 HMS01252snail (sna)VALIUM20mat-tub-Gal4NoNo
 HMS00794wingless (wg)VALIUM20mat-tub-Gal4NoNo
 HMS00844wingless (wg)VALIUM20mat-tub-Gal4NoNo
 HMS01109zerknult 1 (zen1)VALIUM20mat-tub-Gal4NoNo
 HMS01124zerknult 2 (zen2)VALIUM20mat-tub-Gal4NoNo
 HMS00545outstretched (os)VALIUM20mat-tub-Gal4NoNo
 HMS01316tailless (tll)VALIUM20mat-tub-Gal4NoNo
 HMS00922paired (prd)VALIUM20mat-tub-Gal4NoNo
 HMS01443teashirt (tsh)VALIUM20mat-tub-Gal4NoNo
 JF02455decapentaplegic (dpp)VALIUM20mat-tub-Gal460%, retraction defects100%, ventralized
Mat&Zyg genes
 HMS01414armadillo (arm)VALIUM20mat-tub-Gal4100%, segment polarityNT
 HMS01414armadillo (arm)VALIUM20mat-tub-Gal4No100%, segment polarity
(reverse cross)
 HMS00009Notch (N)VALIUM20mat-tub-Gal495%, neurogenic embryosNT
 HMS00009Notch (N) (reverse cross)VALIUM20mat-tub-Gal4NoNT
 GL00092Notch (N)VALIUM22mat-tub-Gal410%, neurogenic embryos75%, neurogenic
 HMS00647domeless (dome)VALIUM20mat-tub-Gal4No90%, JAK/STAT variable
 HMS01293domeless (dome)VALIUM20mat-tub-Gal4100%, head defectsNT
 HMS00856rheaVALIUM20mat-tub-Gal4100%, cuticles WTNT
 HMS00799rheaVALIUM20mat-tub-Gal4100%, cuticles WT100%, dorsal cuticle defects
 HMS00239canoe (cno)VALIUM20mat-tub-Gal4No100%, dorsal open
 GL00633canoe (cno)VALIUM22mat-tub-Gal4No100%, dorsal open
 GL01321hunchback (hb)VALIUM22mat-tub-Gal4No90% head defect, some segmentation defects
 HMS00743upheld (up)VALIUM20mat-tub-Gal4100%, head defects, segments compressedNT
 HMS00076Helicase at 25E (Hel25E)VALIUM20mat-tub-Gal4Many dead embryo with WT cuticle/dead L1 dead/few adults100%, abnormal oogenesis
 HMS00187Proteasome beta3 subunit (Prosbeta3)VALIUM20mat-tub-Gal4Some brown eggs, mostly larvae lethal, very few adultsNT
 HMS00526Not1VALIUM20mat-tub-Gal4Larval lethal, very few adultsNT
 HMS00043myospheroid (mys)VALIUM20mat-tub-Gal485%, variable cuticles, few larvae, few adultsNT
 HMS00310pasilla (pas)VALIUM20mat-tub-Gal485%, cuticles WT, few larvae, very few adultsNT
 HMS01417tumbleweed (tum)VALIUM20mat-tub-Gal480%, cuticles WT, few larvae, very few adultsNT
 HMS00274small nuclear ribonucleoprotein 70K (snrp70K)VALIUM20mat-tub-Gal480%, variable, few larvae, very few adultsNT
 HMS00693shotgun (shg)VALIUM20mat-tub-Gal480%, some dorsal closure defects, few larvae, few adultsNT
 HMS00580trithorax (trx)VALIUM20mat-tub-Gal4Few dead embryos, cuticle WT, larval lethality, few adultsNT
 HMS01009Sirt6VALIUM20mat-tub-Gal480%, cuticles WT, few larvae, no adultsNT
 HMS00968Ribosomal protein S15Aa (Rps15Aa)VALIUM20mat-tub-Gal480%, cuticles WT, few larvae, very few adultsNT
 HMS00084cactus (cac)VALIUM20mat-tub-Gal4No100%, ventralized
 GL00627cactus (cac)VALIUM22mat-tub-Gal4No100%, ventralized
 HMS00317α Catenin (a-Cat)VALIUM20mat-tub-Gal4No99%, blobbed or segment polarity
 HMS01662PDGF- and VEGF-receptor related (Pvr)VALIUM20mat-tub-Gal4No100%, of embryos unhatched, WT cuticle
 HMS00276split ends (spen)VALIUM20mat-tub-Gal4No99%, of embryos with U-shaped and head defects
 HMS00105gawky (gw)VALIUM20mat-tub-Gal4No100%, abnormal oogenesis, fused filaments, a few brown eggs
 HMS00079glorund (glo)VALIUM20mat-tub-Gal4No95%, some JAK/STAT
 HMS00352RhoGAP19DVALIUM20mat-tub-Gal4No50% embryos anterior holes
 HMS00810capulet (capt)VALIUM20mat-tub-Gal4No99%, some U-shaped
 HMS00318Chromosome-associated protein (Cap)VALIUM20mat-tub-Gal4No100%, mostly blobbed
 GL00047Autophagy-specific gene 1 (Atg1)VALIUM22mat-tub-Gal4No80%, some head defects
 HMS00256Mediator complex subunit 25 (Med 25)VALIUM20mat-tub-Gal4No95%, some blobbed, some U-shaped
 HMS00012corkscrew (csw)VALIUM20mat-tub-Gal4No100%, weak corkscrew
 HMS01618zipper (zip)VALIUM20mat-tub-Gal4No100%, abnormal oogenesis, few abnormal eggs
 HMS00035Signal-transducer and activator of transcription protein at 92E (Stat92E)VALIUM20mat-tub-Gal4No100%, JAK/STAT phenotype
 HMS00238connectorVALIUM20mat-tub-Gal4No80%, weak terminal class
enhancer of ksr (cnk)
 HMS00087Histone deacetylase 3 (Hdac3)VALIUM20mat-tub-Gal4100%, head defects100%, ventralized
 HMS00149Son of sevenless (sos)VALIUM20mat-tub-Gal4No100%, weak terminal class
 JF02287discs large 1 (dlg1)VALIUM20mat-tub-Gal4No100%, blobbed and dorsal open
 HMS00111archipelago (ago)VALIUM20mat-tub-Gal4No50%, pair rule phenotype
 HMS00284Ubiquitin-63E (Ubi-63E)VALIUM20mat-tub-Gal4No100%, No development
 HMS00052cap binding protein 80 (cpb80)VALIUM20mat-tub-Gal4100% cuticles WTNo eggs
 HMS00128Elongin CVALIUM20mat-tub-Gal4No100%, white eggs
 HMS00802lethal (2) NC136VALIUM20mat-tub-Gal4100% cuticles WTNo eggs
(l(2)NC136)
 HMS00145Downstream of raf1 (Dsor1)VALIUM20mat-tub-Gal4No100%, terminal defects
 HMS00173rolled (rl)VALIUM20mat-tub-Gal4No100%, blobbed, terminal defects
 GL00215rolled (rl)VALIUM22MTD-Gal4No100%, blobbed, terminal defects
 HMS02519Kruppel (Kr)VALIUM20-miR92amat-tub-Gal4WTWT
 HMS02518Notch (N)VALIUM20-miR92amat-tub-Gal4Neurogenic100% neurogenic
 HMS02520armadillo (arm)VALIUM20-miR92amat-tub-Gal4Segment polarityNT
 HMS02521bicoid (bcd)VALIUM20-miR92amat-tub-Gal4NT100%, bicoid
 HMS02522wingless (wg)VALIUM20-miR92amat-tub-Gal4WTWT
 HMS02516giant (gt)VALIUM20-miR275mat-tub-Gal4NTWT
 HMS02517ovarian tumor (otu)VALIUM20-miR275mat-tub-Gal4NTFew eggs
 HMS02511bicoid (bcd)VALIUM20-miR275mat-tub-Gal4NT100%, bicoid
  • Unless indicated as “reverse cross” maternal-Gal4 females were crossed to shRNA males. F1 maternal-Gal4>>UAS-shRNA females were crossed to sibling males heterozygous for the UAS-shRNAs. %, the fraction of unhatched eggs; no, embryos have normal viability; NT, not tested.