Table 4  Spindle orientation and cell cycle phenotypes of two-cell par embryos treated with RNAi for the indicated genes at semipermissive temperatures
Genotype
par-1(zu310), 19°par-4(it57), 17°par-2(it5), 16°
RNAi% with P1 transverse spindle (N)% with AB/P1 synchrony (N)% with P1 transverse spindle (N)% with AB/P1 synchrony (N)% with P1 transverse spindle (N)% with AB/P1 synchrony (N)
L44400 (30)40 (25)0 (27)18 (27)4 (45)18 (45)
par-165 (23)100** (22)6 (16)100** (16)100** (25)100** (25)
par-40 (30)100** (30)0 (12)83** (12)100** (8)100** (8)
strd-10 (31)97** (31)0 (25)89** (19)56** (30)63** (30)
pig-10 (19)74* (19)88** (27)100** (26)
mes-14 (26)61* (23)0 (18)13 (18)
ntl-90 (25)32 (25)83** (24)78** (23)
gei-174 (26)48 (25)0 (17)18 (17)
nop-12 (41)54 (41)100** (19)100** (19)
math-334 (24)41 (22)0 (17)20 (15)100** (29)97** (29)
uba-20 (16)43 (16)0 (25)5 (22)15* (27)22 (27)
sti-10 (38)55** (38)
kin-100 (27)11 (27)
lgl-1100** (23)96** (25)
  • RNAi clones producing >60% embryonic lethality in the par mutant at semipermissive temperature were used for this analysis. —, lethality was <60%. Percentages are rounded to the nearest whole number. *P < 0.05, χ2 test; **P < 0.0001, χ2 test.