Table 1  Percentage of embryonic lethality in conditional par mutants treated with RNAi of screen positives
Genotype
RNAi gene targetFunctionN2 (19°)par-1(zu310) (19°)par-4(it57) (17°)
 L4440 (negative control)RNAi feeding vector0.8 ± 0.614.0 ± 1.626.7 ± 13.8
sad-1 (negative control)AMPK-related kinase, neuronal polarity0.6 ± 0.219.0 ± 7.221.0 ± 9.5
par-4 (positive control)PAR-4 kinase, embryo cell polarity26.2 ± 8.9**84.8 ± 5.2**87.1 ± 1.9**
Phosphorylation
strd-1PAR-4 kinase cofactor0.6 ± 0.498.4 ± 1.6**90.2 ± 6.2**
pig-1AMPK-related kinase, neuronal polarity1.8 ± 0.4*95.7 ± 3.6**40.8 ± 7.2
kin-10Casein Kinase II regulatory subunit26.3 ± 8.3**36.8 ± 2.4a68.8 ± 4.5*
ZK856.8Calcium-binding phosphatase-like0.4 ± 0.238.1 ± 9.1*11.3 ± 3.8
Cytoskeletal and membrane proteins
mes-1Membrane protein, embryo cell polarity0.7 ± 0.188.0 ± 2.6**64.0 ± 17.5*
unc-59Septin GTPase2.2 ± 0.3*54.0 ± 6.2**43.7 ± 9.7
lgl-1Cell polarity0.7 ± 0.451.1 ± 2.2*23.5 ± 4.0
T24F1.2Membrane protein1.6 ± 1.334.5 ± 3.7**52.0 ± 6.7*
npp-22Nucleoporin0.9 ± 0.833.6 ± 5.2*49.8 ± 17.2
str-43Membrane protein0.8 ± 0.329.9 ± 6.9*55.2 ± 15.9
ooc-3ER membrane protein, cell polarity0.8 ± 0.424.6 ± 4.4*16.1 ± 6.1
Ubiquitination, sumoylation
math-33USP7/HAUSP ubiquitin hydrolase1.9 ± 1.275.3 ± 6.3**89.6 ± 6.1*
uba-2SUMO-activating protein24.4 ± 2.6**74.9 ± 19.8*84.1 ± 7.2*
gei-17SUMO ligase5.0 ± 1.2**81.1 ± 0.8**83.7 ± 4.5*
Chaperones
sti-1HSP90/HSP70 co-chaperone0.9 ± 0.44.9 ± 0.5***88.4 ± 11.4*
hsp-2Pseudogene, also targets HSP-12.8 ± 1.717.5 ± 0.758.7 ± 16.6*
Unknown function
ntl-9Rcd1 superfamily1.0 ± 0.487.4 ± 3.3**49.8 ± 1.9
F25B5.2 (nop-1)Unknown; nematode specific0.8 ± 6.880.1 ± 11.0*44.0 ± 7.2
F23F12.3 intronUnknown; secondary targets0.6 ± 0.176.0 ± 6.3**39.9 ± 0.9
  • Percentage of embryo lethality ± SD from mean following RNAi feeding of clones corresponding to the indicated genes. *P < 0.05, enhanced lethality, compared to L4440 control in the same genetic background. **P < 0.001, enhanced lethality, compared to L4440 control. ***P < 0.01, suppression of lethality, compared to L4440 control.

  • a Not significantly different from result in N2, though lethality is increased compared to L4440 in par-1(zu310).