Table 2  Percentage of embryo lethality in par-2(it5ts) and par-3(e2074); sup-7 treated with RNAi of enhancers of par-1 and par-4 embryo lethality
RNAi gene targetpar-2(it5)aPar-3(e2074); sup-7b
L4440 (negative control)9.0 ± 1.956.5 ± 11.1
sad-1 (negative control)16.0 ± 5.858.5 ± 15.1
par-4 (positive control)61.8 ± 11.3**97.5 ± 1.4**
strd-179.6 ± 1.3**65.8 ± 9.3
pig-196.5 ± 0.1**69.5 ± 15.8
mes-128.7 ± 19.370.5 ± 13.0
ntl-981.0 ± 12.5**89.4 ± 4.6**
gei-1742.1 ± 3.7*81.0 ± 7.5*
F25B5.2 (nop-1)99.4 ± 0.4**79.8 ± 3.5*
F23F12.3 intron45.9 ± 15.1*69.0 ± 5.3
math-3399.1 ± 1.3**33.2 ± 7.6***
uba-271.9 ± 6.9*94.2 ± 5.3**
unc-5915.2 ± 7.162.5 ± 16.5
lgl-198.9 ± 0.1**25.9 ± 13.2***
ZK856.823.4 ± 6.466.2 ± 13.0
kin-1023.9 ± 12.084.4 ± 39.0d
T24F1.250.6 ± 5.6*62.0 ± 5.0
npp-2257.0 ± 8.6*54.3 ± 14.1
str-4311.1 ± 5.658.1 ± 17.2
ooc-328.7 ± 15.664.9 ± 14.2
hsp-221.1 ± 9.366.8 ± 7.5
sti-129.3 ± 12.7*77.5 ± 9.4*
  • Percentage of embryo lethality ± SD from mean following RNAi feeding of clones corresponding to the indicated genes, ordered by degree of lethality produced in par-1(zu310). *P < 0.05, enhanced lethality, compared to L4440 control in the same genetic background. **P < 0.001, enhanced lethality, compared to L4440 control. ***Suppression of lethality, P < 0.05, compared to L4440 control.

  • a 16°.

  • b 22°.

  • c Not significantly different from expected lethality produced by RNAi in wild type added to control levels of lethality.