Table 3 Interallelic complementation between Top2 mutants y1w67c23/Y; Top2m/CyO, y+ × y1w67c23/ y1w67c23; Top2m/CyO, y+
Allele transmitted by female
Allele transmitted by male17-1 WHD17-3 TOPRIM17-6 TOPRIM35-1a TOPRIM35-5 ATPase35-13 WHD
17-10b (165)c19 (275)13 (354)56 (612)95 (217)0 (220)
WHD
17-322 (932)2 (163)64 (311)22 (719)93 (323)77 (183)
TOPRIM
17-626 (214)52 (243)0 (248)58 (708)3 (147)76 (208)
TOPRIM
35-146 (557)31 (705)71 (627)0 (98)78 (226)85 (360)
TOPRIM
35-585 (220)88 (440)2 (777)86 (346)0 (103)64 (426)
ATPase
35-130 (184)60 (350)55 (268)79 (655)98 (240)0 (275)
WHD
  • Boldface type, 11–100% viability. Vials were scored daily.

  • a Top235-1 has three amino acid changes; however, only one change is in an invariant amino acid (P465S in TOPRIM domain).

  • b Percentage of viability is the number of Cy+ flies divided by half the number of Cy flies multiplied by 100.

  • c Total number of Cy flies scored.