Table 1  rtn1Δ yop1Δ cells have mild SPB positioning defects upon α-factor arrest
Wild typertn1Δ yop1Δ
Microtubules positioned in shmoo335 (92.6%)384 (87.3%)
Microtubules positioned away from shmoo27 (7.4%)56 (12.6%)
Total362440
  • Parental (YOL183) or rtn1Δ yop1Δ (SWY3811) cells expressing GFP-Tub3 arrested with α-factor. Cells were fixed to preserve GFP fluorescence and imaged and scored based on proximity of SPB and microtubules to the shmoo; P-value= 0.00012.