TABLE 1

Monte Carlo simulation of mutation, bottlenecks, and selection in serial transfer culture

Dilution factord = 10−3d = 10−4d = 10−5d = 10−6
10 ml
Fixed for n0098 (37.4 ± 3.8)53 (29.8 ± 4.2)20 (32.7 ± 7.5)0 (—)
Fixed for n11324 (44.0 ± 30.5)356 (33.7 ± 1.8)118 (34.3 ± 3.2)28 (34.1 ± 6.7)
Polymorphic7891362472
100 ml
Fixed for n00371 (41.2 ± 2.1)193 (34.3 ± 2.5)53 (29.1 ± 4.1)14 (22.9 ± 6.6)
Fixed for n113 (44.0 ± 30.5)273 (29.7 ± 1.8)423 (27.8 ± 1.3)195 (31.2 ± 2.3)
Polymorphic1263424291
  • Number of runs initiated solely with n10 that are fixed for either n00 or nil, or polymorphic at the 50th transfer (italic), and the mean and standard deviation of the transfer at which fixation occurred. The resource concentration, population growth, and mutation and selection parameters used in these simulations are identical to those in the legend to Figure 3. These runs terminated at the end of the 50th transfer or when, at the end of an earlier transfer, n00 was fixed (n10 = n01 = n11 = 0) or nil was fixed (n00 = n10 = n01 = 0).