Table 1 Localizarion of SNB-1 in mutants related to neuronal polarity, synapse formation, or inositol metabolism
Strain usedMutationGeneLocalization of snb-1
N2Wild typePresynaptic region
IK589ttx-7(nj50)IMPaseEntire process
Polarity- or synapse-related genes
KU17lrk-1(km17)aLRRK2/PARK8-related kinaseWT
VC898cdc-42(gk388)b,cCdc42WT
NG324wsp-1(gm324bWASPWT
VC2053wip-1(ok2435)b,cWASP-interacting proteinWT
VC2706wve-1(ok3308)b,cWAVEWT
DR1unc-101(m1)AP-1 medium subunitWT
DR1ttx-7(nj40) unc-101(m1)AP-1 medium subunitttx-7-like
CB1193unc-33(e1193)CRMPWT
CB204unc-33(e204)CRMPWT
SP1382unc-33(mn407)CRMPWT
EM67mab-20(bx24)aSemaphorin-2AWT
CB78unc-6(e78)aNetrinWT
CB271unc-40(e271)aNetrin receptorWT
CB362unc-44(e362)bAnkyrin GWT
Inositol metabolism-related genes
CB205unc-26(e205)SynaptojaninWT (Figure 4)
CB205ttx-7(nj50);unc-26(e205)SynaptojaninWT (Figure 4)
RB1535arf-1.1(ok1840)ADP-ribosylation factor familyWT
VC567arf-1.2(ok796)ADP-ribosylation factor familyWT
FX1447arf-6(tm1447)bADP-ribosylation factor familyWT
KU22pld-1(km22)Phospholipase DWT
VC1587ocrl-1(gk752)OCRLWT
VC1587ttx-7(nj40) ocrl-1(gk752)OCRLttx-7-like
IK1130age-1(mg305)Phosphoinositide 3-kinaseWT
IK1130ttx-7(nj50);age-1 (mg305)Phosphoinositide 3-kinasettx-7-like
RB1813piki-1(ok2346)Phosphoinositide 3-kinaseWT
KR1440vps-34(h797)b,cPhosphoinositide 3-kinaseWT
FX2348F35H12.4(tm2348)bPhosphatidylinositol kinaseWT
VC2563Y75B8A.24(ok3320)bPhosphatidylinositol kinaseWT
EG3361ttx-7(nj50) gpIs25[rab-3p::ppk-1]Type I PI-4-phosphate kinasettx-7-like
FX3741ppk-2(tm3741)bType II PI -5-phosphate kinaseWT
MT7531ppk-3(n2835)bType III PI-3-phosphate kinaseWT
MT12352trr-1(n3630)b,cTRAAP subfamilyWT
TR1331smg-1(r861)bPhosphatidylinositol kinaseWT
VC381atm-1(gk186)bATM familyWT
VC728atl-1(ok1063)bATM familyWT
VC2312let-363(ok3013)b,cFRAP1WT
FX753plc-1(tm753)Phospholipase cWT
FX753ttx-7(nj40);plc-1 (tm753)Phospholipase cttx-7-like
RB1496plc-2(ok1761)bPhospholipase cWT
RB1496ttx-7(nj50);plc-2(ok1761)bPhospholipase cttx-7-like
MT1434egl-30(n686)G-protein α-subunitWT
KY26egl-30(tg26gf)G-protein α-subunitWT
MT1434egl-30(n686) ttx-7(nj50)G-protein α-subunitttx-7-like
  • Localization of SNB-1 in RIA neuron was examined. About 10 adult animals were examined in more than three trials for each genotype. In the mutants without the superscripts, the localization of SNB-1::VENUS expressed from integrated array was observed. WT and ttx-7-like represents the wild-type and ttx-7-like mutant phenotypes of SNB-1 localization, respectively.

  • a The localization of SNB-1::DsRedmonomer expressed from integrated array was observed.

  • b The localization of SNB-1::VENUS expressed from extrachromosomal array was observed.

  • c These mutants display larval arrest or developmental defects. The localization of SNB-1 in these mutants was examined at larval stages; SNB-1 localized to the distal region of the process in wild-type animals at laval stages as well as at the adult stage.