Table 2  APL-1 overexpression causes a temperature-sensitive lethality
20°25°27°
Strain (genotype)NeggsNwormsTAverage % survival STE% L1NeggsNwormsTAverage % survival STE% L1NeggsNwormsTAverage % survival STE% L1
Wild type (N2)3462357230103 ± 0.70131013519104 ± 3.005633600650106 ± 1.61
ynIs107 [APL-1(yn32)D342C/S362C::GFP]5185284102 ± 1.408108168100 ± 1.91
ynIs100 [apl-1(yn32)::GFP]3863944102 ± 2.305756216106 ± 2.31
ynIs106 [apl-1(yn5 yn32)]256259398 ± 10.703493523101 ± 1.10316311398 ± 1.40
ynIs86 [APL-1]455404588 ± 4.5 *0420315477 ± 4.7 *0449265460 ± 3.5 *31
ynIs79 [APL-1::GFP]10156391063 ± 5.6 *1494266534 ± 10.8 *3169064144.3 ± 0.9 *47
ynIs71 [APL-1EXT]488397682 ± 4.5 *1283180364 ± 8.6 *9484261554 ± 2.9 *72
apl-1(yn5)9438261186 ± 1.7 *2694512675 ± 2.1 *4373718414147 ± 1.9 *46
apl-1(yn5); yn38668635595 ± 4.82497480596 ± 1.4 **23
apl-1(yn5); yn39364237369 ± 10.9 *,**8586117518 ± 2.9 *,**69
  • Is alleles indicate integrated transgenes. Transgenes shown in brackets. Transgenes were driven by the apl-1 promoter unless otherwise noted; the encoded protein is indicated; DAF-16::GFP is driven by the daf-16 promoter. apl-1(yn32) is a missense mutation that corresponds to a null allele. N = number of animals observed. T = number of independent times or trials experiment was performed. SUR-5::GFP was used as a co-injection marker for ynIs71 and ynIs86, Pmyo-2::GFP for ynIs106 and ynIs107, and rol-6(su1006) for ynIs79 and ynIs100. For statistical analysis: P-values were determined by one-way ANOVAs with Tukey post-test (95% confidence intervals) for survival rate and are only indicated if P < 0.001 against wild type (*) or apl-1(yn5) (**) at the given temperature. STE, standard error.