Table 1 Yeast strains used in this study
StrainGenotypeSourceApplication
ZLY2254MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 lst8::kanMX4 ade2Δ::HIS3 [pRS412-LST8]This study
ZLY3081 (WT)MATα ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 lst8::kanMX4 ade2Δ::HIS3 [pRS412-LST8]This studyFigure 1
TPY104 (leb1)MATα ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 lst8::kanMX4 ade2Δ::HIS3 leb1::Tn3::LEU2 [pRS412-LST8]This studyFigure 1
TPY103 (leb2)MATα ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 lst8::kanMX4 ade2Δ::HIS3 leb2::Tn3::LEU2 [pRS412-LST8]This studyFigure 1
ZLY423 (WT)MATa ade2-1 ura3 his3-11,15 leu2 lst8::LEU2 [pRS412-LST8]This studyFigure 2A
TPY115 (far11 lst8)MATa ade2-1 ura3 his3-11,15 leu2 lst8::LEU2 far11::kanMX4This studyFigure 2A
TPY114 (far11)MATa ade2-1 ura3 his3-11,15 leu2 lst8::LEU2 far11::kanMX4 [pRS412-LST8]This studyFigure 2A
TPY122 (fpr1)MATa ade2-1 ura3 his3-11,15 leu2 lst8::LEU2 fpr1::kanMX4 [pRS412-LST8]This studyFigure 2A
ZLY2405 (sac7 lst8)MATa ade2-1 ura3 his3-11,15 leu2 lst8::LEU2 sac7::kanMX4This studyFigure 2A
ZLY2404 (sac7)MATa ade2-1 ura3 his3-11,15 leu2 lst8::LEU2 sac7::kanMX4 [pRS412-LST8]This studyFigure 2, A and B
ZLY2845 (sac7 lst8)MATa ade2-1 ura3 his3-11,15 leu2 lst8::LEU2 sac7::kanMX4 [pRS412]This studyFigure 2B
TWY680MATa AVO3-GFP::kanR ura3 trp1 leu2 his3 ade2 can1-100Berchtold and Walther (2009)
TWY696MATa BIT61-GFP::HIS ura3 trp1 leu2 his3 ade2 can1-100
TWY748MATa KOG1-GFP::HIS ura3 trp1 leu2 his3 ade2 can1-100
TPY1264 (WT, no GFP)MATa sac7::kanMX4 far11::TRP1 ura3 leu2 his3 ade2 trp1 can1-100This studyFigure 2C
TPY1266 (lst8, no GFP)MATa sac7::HIS3 far11::TRP1 lst8::LEU2 ura3 leu2 his3 ade2 trp1 can1-100This studyFigure 2C
TPY369 (AVO3-GFP)MATa AVO3-GFP::kanR ura3 trp1 leu2 his3 ade2 can1-100 sac7::HIS3 far11::TRP1This studyFigure 2
TPY407 (lst8 AVO3-GFP)MATa AVO3-GFP::kanR ura3 trp1 leu2 his3 ade2 can1-100 sac7::HIS3 far11::TRP1 lst8::LEU2This studyFigure 2
TPY358 (BIT61-GFP)MATa BIT61-GFP::HIS ura3 trp1 leu2 his3 ade2 can1-100 sac7::kanMX4 far11::TRP1This studyFigure 2C
TPY366 (lst8 BIT61-GFP)MATa BIT61-GFP::HIS ura3 trp1 leu2 his3 ade2 can1-100 sac7::kanMX4 far11::TRP1 lst8::LEU2This studyFigure 2C
TPY371 (KOG1-GFP)MATa KOG1-GFP::HIS ura3 trp1 leu2 his3 ade2 can1-100 sac7::kanMX4 far11::TRP1This studyFigure 2C
TPY413 (lst8 KOG1-GFP)MATa KOG1-GFP::HIS ura3 trp1 leu2 his3 ade2 can1-100 sac7::kanMX4 far11::TRP1 lst8::LEU2This studyFigure 2C
SY2227MATa ade1-1 leu2-2,113 trp1 ura3-52 bar1 HIS3::pFUS1::HIS3 mfa2-Δ1::FUS1-lacZ rad16::pGAL1::STE4Kemp and Sprague (2003)
SY4078SY2227 FAR7-myc13-KAN <pSL2771>
SY4079SY2227 FAR8-myc13-KAN <pSL2771>
SY4080SY2227 FAR9-myc13-KAN <pSL2771>
SY4081SY2227 FAR10-myc13-KAN <pSL2771>
SY4082SY2227 FAR11-myc13-KAN <pSL2771>
TPY978SY2227 FAR7-myc13-KAN [pRS416-FAR11-HA]This studyFigure 3A
TPY981SY2227 FAR8-myc13-KAN [pRS416-FAR11-HA]This studyFigure 3A
TPY1001SY2227 FAR9-myc13-KAN [pRS416-FAR11-HA]This studyFigure 3A
TPY1002SY2227 FAR10-myc13-KAN [pRS416-FAR11-HA]This studyFigure 3A
TPY1003SY2227 FAR11-myc13-KAN [pRS416-FAR11-HA]This studyFigure 3A
RBY231 (WT)MATα ura3 leu2 lys2 [pRS416-LST8]This studyFigure 3B, Figure 5B
RBY223 (lst8)MATα ura3 leu2 lys2 lst8::LEU2 [pRS416-LST8]This studyFigure 3B, Figure 5B
MOY142 (lst8 far3)MATα ura3 leu2 lys2 lst8::LEU2 far3::kanMX4 [pRS416-LST8]This studyFigure 3B
MOY145 (lst8 far7)MATα ura3 leu2 lys2 lst8::LEU2 far7::kanMX4 [pRS416-LST8]This studyFigure 3B
MOY146 (lst8 far8)MATα ura3 leu2 lys2 lst8::LEU2 far8::kanMX4 [pRS416-LST8]This studyFigure 3B
MOY169 (lst8 far9)MATα ura3 leu2 lys2 lst8::LEU2 far9::kanMX4 [pRS416-LST8]This studyFigure 3B
MOY149 (lst8 far10)MATα ura3 leu2 lys2 lst8::LEU2 far10::kanMX4 [pRS416-LST8]This studyFigure 3B
MOY150 (lst8 far11)MATα ura3 leu2 lys2 lst8::LEU2 far11::kanMX4 [pRS416-LST8]This studyFigure 3B
SH100 (WT)MATa leu2-3,112 ura3-52 rme1 trp1 his4 HMLa ade2 tor2::ADE2 [YCplac111::TOR2]Helliwell et al. (1998a)Figure 3C, Figure 4, Figure 5D, Figure 6A–C
SH121 (tor2-21)MATa leu2-3,112 ura3-52 rme1 trp1 his4 HMLa ade2 tor2::ADE2 [YCplac111::tor2-21]Figure 3C, Figure 4, Figure 5D, Figure 6, B and C
SH221 (tor1 tor2-21)MATa leu2-3,112 ura3-52 rme1 trp1 his3 HMLa ade2 tor1::HIS3 tor2::ADE2 [YCplac111::tor2-21]
TPY157 (tor2-21 far3)SH121 far3::kanMX4This studyFigure 3C
TPY147 (tor2-21 far7)SH121 far7::kanMX4This studyFigure 3C
TPY213 (tor2-21 far8)SH121 far8::kanMX4This studyFigure 3C
TPY207 (tor2-21 far9)SH121 far9::kanMX4This studyFigure 3C
TPY151 (tor2-21 far10)SH121 far10::kanMX4This studyFigure 3C
TPY116 (tor2-21 far11)SH121 far11::kanMX4This studyFigure 3C, Figure 4C, Figure 6B
TPY110 (tor2-21 sac7)SH121 sac7::kanMX4This studyFigure 4A, Figure 4C, Figure 6B
TPY311 (tor2-21 far11)SH121 far11::TRP1This studyFigure 4, C and D
TPY301 (tor2-21 sac7 far11)SH121 sac7::kanMX4 far11::TRP1This studyFigure 4C
TPY680 (tor2-21 far11 rom2)SH121 rom2::kanMX4 far11::TRP1This studyFigure 4D
BY4741MATa ura3 leu2 his3 met15Yeast Genome Deletion Project
BY4741 far11BY4741 far11::kanMX4Figure 5A
TPY633BY4741 tpd3::kanMX4 far11::HIS3This studyFigure 5A
TPY632BY4741 pph21::kanMX4 pph22::kanMX4 far11::HIS3This studyFigure 5A
TPY625 (lst8 tpd3)MATα ura3 leu2 lys2 lst8::LEU2 tpd3::kanMX4 [pRS416-LST8]This studyFigure 5B
TPY648 (lst8 rts1)MATα ura3 leu2 lys2 lst8::LEU2 rts1::kanMX4 [pRS416-LST8]This studyFigure 5B
TPY732 (lst8 cdc55)MATα ura3 leu2 lys2 lst8::LEU2 cdc55::kanMX4 [pRS416-LST8]This studyFigure 5B
BY4741 (WT)MATa ura3 leu2 his3 met15 [pRS416]This studyFigure 5C
BY4741 (lst8)MATa ura3 leu2 his3 met15 lst8::LEU2 [pRS416-LST8]This studyFigure 5C
BY4741 (pph21/22)MATa ura3 leu2 his3 met15 pph21::kanMX4 pph22::kanMX4[pRS416-LST8]This studyFigure 5C
TPY622 (lst8 pph21/22)MATa ura3 leu2 his3 met15 lst8::LEU2 pph21::kanMX4 pph22::kanMX4[pRS416-LST8]This studyFigure 5C
TPY665 (rts1)MATa leu2-3,112 ura3-52 rme1 trp1 his4 HMLa ade2 tor2::ADE2 rts1::kanMX4 [YCplac111::TOR2]This studyFigure 5D, Figure 6C
TPY601 (tor2-21 rts1)MATa leu2-3,112 ura3-52 rme1 trp1 his4 HMLa ade2 tor2::ADE2 rts1::kanMX4 [YCplac111::tor2-21]This studyFigure 5D, Figure 6C
TB50aMATa leu2–3,112 ura3–52 trp1 his3 rme1 HMLaWullschleger et al. (2005)Figure 7
SW70TB50a 3HA-TOR2Figure 7
TPY1246TB50a 3HA-TOR2 sac7::HIS3This studyFigure 7
TPY1249TB50a 3HA-TOR2 far11::TRP1This studyFigure 7