Table 2  ztf-16 acts in glia to control ver-1 expression
% adult animals expressing ver-1 in AMsh glia at:
Genotypea15°25°
Wild type093
ztf-16(ns171)016
ztf-16(ns171); glia::ztf-16ab064c
ztf-16(ns171); glia::ztf-16b080c
ztf-16(ns171); embryonic glia::ztf-16bdND10
ztf-16(ns169)04
ztf-16(ns169); glia::ztf-16a044e
ztf-16(ns169); glia::ztf-16b060c
  • Transgenes were injected at 60 ng/μl of the rescuing plasmid, with 60 ng/μl of pRF4. Lines shown are nsEx1389, nsEx1410, nsEx3266, nsEx1382, and nsEx1405 and are representative of others. n > 25 for all strains. ND, not determined.

  • a All strains contained the ver-1 promoter::gfp transgene (nsIs22).

  • b The glia promoter (F16F9.3) drives expression in the AMsh and PHsh glia (Bacaj et al. 2008). However, rescue of ver-1 promoter::gfp expression in the PHsh glia was not observed (data not shown), perhaps due to low expression levels.

  • c P < 0.001. P-values were determined using the χ2 test. Rescue lines were compared against the corresponding mutant alone.

  • d The glial enhancer element from the lin-26 promoter (Landmann et al. 2004) was used to drive expression in embryonic glial cells.

  • e P < 0.01. P-values were determined using the χ2 test. Rescue lines were compared against the corresponding mutant alone.