| % adult animals expressing ver-1 in AMsh glia at: | ||
|---|---|---|
| Genotypea | 15° | 25° |
| Wild type | 0 | 93 |
| ztf-16(ns171) | 0 | 16 |
| ztf-16(ns171); glia::ztf-16ab | 0 | 64c |
| ztf-16(ns171); glia::ztf-16b | 0 | 80c |
| ztf-16(ns171); embryonic glia::ztf-16bd | ND | 10 |
| ztf-16(ns169) | 0 | 4 |
| ztf-16(ns169); glia::ztf-16a | 0 | 44e |
| ztf-16(ns169); glia::ztf-16b | 0 | 60c |
Transgenes were injected at 60 ng/μl of the rescuing plasmid, with 60 ng/μl of pRF4. Lines shown are nsEx1389, nsEx1410, nsEx3266, nsEx1382, and nsEx1405 and are representative of others. n > 25 for all strains. ND, not determined.
↵a All strains contained the ver-1 promoter::gfp transgene (nsIs22).
↵b The glia promoter (F16F9.3) drives expression in the AMsh and PHsh glia (Bacaj et al. 2008). However, rescue of ver-1 promoter::gfp expression in the PHsh glia was not observed (data not shown), perhaps due to low expression levels.
↵c P < 0.001. P-values were determined using the χ2 test. Rescue lines were compared against the corresponding mutant alone.
↵d The glial enhancer element from the lin-26 promoter (Landmann et al. 2004) was used to drive expression in embryonic glial cells.
↵e P < 0.01. P-values were determined using the χ2 test. Rescue lines were compared against the corresponding mutant alone.