Table 1  Mutant strains that reduce ver-1 promoter::gfp expression
ver-1 expression 25°a
Allelea% AMsh onn
Wild type9860
Complementation group 1 (see also dominant alleles below)
ttx-1(p767) (reference allele)b040
ns235064
ns252062
Complementation group 2
ns258 (reference allele)b652
ns16772c54
ns1701754
ns174544
ns2341655
ns23769c51
ns238445
ns2413152
ns249245
ns2684560
Complementation group 3
ns171 (reference allele)b455
ns1691451
ns178252
Alleles not falling into complementation groups 1–3
ns23148c52
ns25770c50
Dominant allelesd
ns255e042
ns259/+f244
ns260/+f4284
ns267040
  • a All strains contained the ver-1 promoter::gfp transgene (nsIs22).

  • b The reference alleles were used to place other alleles into complementation groups.

  • c These alleles mostly had weak, qualitative effects on ver-1 promoter::gfp expression.

  • d Dominant alleles were not scored for complementation. All dominant alleles were found to have a mutation in the ttx-1 gene.

  • e Animals homozygous for allele ns255 were slow growing and unhealthy.

  • f Alleles ns259 and ns260 were homozygous sterile and lethal, respectively. These strains were isolated from the screen as heterozygotes and are scored as such. Of the ns260/+ animals that expressed ver-1 promoter::gfp, fluorescence was qualitatively reduced.