Table 4 Genetic interactions between ppa2-6, ppa2–Δ, ppa1–Δ, ypa1–Δ, ypa2-7, and ypa2–Δ
Mutantppa1−Δppa2−Δppa2-6ypa1−Δypa2−Δypa2-7
ppa1−ΔNDSynthetically lethal: single, small, rounded cellSynthetically lethal: 1 or 2 small rounded cells at 32° and 36°Cold sensitive at 19°, similar to ypa1−ΔCold sensitive at 19°, similar to ypa2−ΔCold sensitive at 19°, similar to ypa2-7
ppa2−ΔNDND (allelic)Cells are wee-like ppa2−Δ at >29° and cold sensitive-like ypa1−Δ at 19°Additive negative effect: cells are cold sensitive at 19°, very slow growing wee at >29°Cells are wee at >25° and cold sensitive at 19°
ppa2-6NDSlow growing colony of rounded, lysed cellsSynthetically lethal: 1–2 rounded cells after germinationSynthetically lethal: 1–2 rounded cells after germination
ypa1−ΔNDSynthetically lethal: cells undergo 1–2 divisions dying as rounded cellsSynthetically lethal: cells undergo 1–2 divisions dying as rounded cells
ypa2−ΔNDND (allelic)
ypa2-7ND
  • Mutants were crossed at 25°. Tetrads were dissected and spores were germinated on complete medium at 32°. In cases were lethal interactions were observed, other temperatures were tested, but no temperature in the range of 19°–36° altered the result obtained. At least 10 tetrads were analyzed for all double mutant combinations. ND, not done.