Table 2 Summary of hatch rate and cuticle analysis for transgenes in the APC2g10 APC1Q8 background
Weak cuticle classStrong cuticle class
rate (%)0–1 (%)1.5–2.5 (%)PA3–4 (%)4.5–6 (%)PACategorya
APC2-FL; APC2g10 APC1Q84645.560.720.5NA1
APC2 R3–R5 SA; APC2g10 APC1Q8683231.860NA1
APC2 R3–R5 SD; APC2g10 APC1Q8405700.530NA1
APC2 R3–R5 ExR; APC2g10 APC1Q8505000.500NA1
APC2 R1–R5 SA; APC2g10 APC1Q85121NA4064.22
APC2 R1–R5 SD; APC2g10 APC1Q84920NA38114.42
APC2 R1–R5 ExR; APC2g10 APC1Q82115161.426224.53
APC2 Δ20RΔB; APC2g10 APC1Q8241240.75824.03
APC2 Δ15RΔ20RΔB; APC2g10 APC1Q81815131.35044.03
APC2g10 APC1Q8231681.242114.33
  • The n for the hatch rate experiments was >100. For cuticle analysis, n > 40. NA, not applicable.

  • a Hatch rate and cuticle phenotype differences within each category are not statistically significant, with the exception of APC2 R3–R5 SA; APC2g10 APC1Q8, which has a significantly higher hatch rate. The hatch rate and cuticle phenotype data are statistically significantly different between categories on the basis of hatch rate, cuticle phenotype, or both. Groups 1 and 2 differ significantly between each other on the basis of cuticle phenotype, but not hatch rate (with the exception noted), whereas groups 1 and 2 both differ significantly from group 3 on the basis of hatch rate and cuticle phenotype. Hatch rate statistics were calculated using the chi-square test, χ2 = 129.5 (d.f. = 9, n = 3739), P < 0.001, followed by the Tukey-type posthoc test, q(d.f. = ∞, groups = 10), P < 0.05 per comparison. The statistical analysis of cuticle phenotypes was performed using the Kruskal–Wallis test, χ2 = 525.7 (d.f. = 9, n = 1197), P < 0.001, followed by the Dunn procedure for multiple comparisons using an adjusted α-level of 0.0006 for a two-tailed test (P = 0.001).