Genotype | Hatch rate (%) | 0–1 (%) | 1.5–2.5 (%) | 3–4 (%) | 4.5–6 (%) | PA | Category^{a} |
---|---|---|---|---|---|---|---|

APC2-FL; APC2^{g10} | 98 | 2 | 0.2 | 1 | |||

APC2 R3-R5 SD; APC2^{g10} | 97 | 3 | <1 | 0.6 | 1 | ||

APC2 R3–R5 SA; APC2^{g10} | 93 | 7 | 0.2 | 2 | |||

APC2 R3–R5 ExR; APC2^{g10} | 91 | 8 | 1 | 0.3 | 2 | ||

APC2 R1–R5 SD; APC2^{g10} | 89 | 11 | <1 | 0.5 | 3 | ||

APC2 Δ20RΔB(low); APC2^{g10} | 85 | 12 | 2 | 1 | 0.9 | 3 | |

APC2 R1–R5 SA; APC2^{g10} | 72 | 18 | <1 | 0.5 | 4 | ||

APC2 Δ15RΔ20RΔB(low); APC2^{g10} | 68 | 15 | 8 | 7 | <1 | 1.8 | 5 |

APC2 Δ20RΔB(high); APC2^{g10} | 52 | 31 | 17 | <1 | 1.1 | 6 | |

APC2 R1–R5 ExR; APC2^{g10} | 34 | 50 | 16 | 1.0 | 7 | ||

APC2 Δ15RΔ20RΔB(high); APC2^{g10} | 36 | 18 | 15 | 31 | 2.4 | 8 | |

APC2^{g10} | 0 | 14 | 6 | 74 | 6 | 3.4 | 9 |

The

*n*for the hatch rate experiments was >200. For cuticle analysis,*n*> 19.↵a Overall, the cuticle phenotypes have a consistent, statistically significant increasing trend in severity as the hatch rate decreases [chi-square test for trend, χ

^{2}= 229.24 (d.f. = 1,*n*= 1033),*P*< 0.001]. The categories were grouped so that genotypes would not differ significantly with respect to hatch rate or cuticle phenotype within each category. Categories 1–9 each have at least one genotype that differs significantly from at least one genotype in every other category, but there is some overlap between genotypes in categories 1–3. For example, the*APC2 R3–R5 SA*hatch rate (from category 2) differs significantly from the*APC2-FL*hatch rate (from category 1), but not the*APC2 R3–R5 SD*hatch rate (category 1). Hatch rate statistics were calculated using the chi-square test, χ^{2}= 2021 (d.f. = 11,*n*= 6175),*P*< 0.001, followed by the Tukey-type posthoc test (d.f. = ∞, groups = 12),*P*< 0.05 per comparison. The statistical analysis of cuticle phenotypes was performed using the Kruskal–Wallis test, χ^{2}= 285.9 (d.f. = 11,*n*= 1033),*P*< 0.001, followed by the Dunn procedure for multiple comparisons using an adjusted α-level of 0.0004 for a two-tailed test (*P*< 0.001).