Table 2  Mutations present in the or683ts interval
PositionaGeneWTbAlterationcNotesdReadse
7,029,617Intergenic*A insertion11
7,047,283Intergenic*C insertion20
7,063,069R01H2.4*C insertionAltered C terminus, NE15
7,214,105R151.2ACfAnnotation error?27
7,228,289R151.8AACDeletionIntron11
7,272,182psd-1*G insertionIntron26
7,297,779B0361.2*AA insertionIntron10
7,318,365F56C9.8*TT insertionIntron11
7,587,395cup-5GDeletionIntron12
7,628,358fbn-1TGDeletionAltered C terminus, E11
7,686,828C18H2.3*TA insertion3′-UTR10
7,696,424C18H2.4TADeletionAltered C terminus, NE12
7,712,933Intergenic*G insertion17
7,781,836mab-5AGDeletionIntron11
7,864,173C02C2.4CDeletionIntron23
7,919,577tag-250*GA insertionAltered C terminus, NE12
7,937,298ran-2TCDeletion3′-UTR11
7,958,664F54H12.3CTTransposon15
8,042,705K12H4.6*G insertionAltered C terminus, NE32
8,053,797IntergenicCDeletion10
8,059,766IntergenicCT21
8,064,605IntergenicCT17
8,068,402ceh-26*G insertionfAnnotation error?11
8,070,697ceh-26*G insertionIntron25
8,075,189dcr-1*GG insertionAltered C terminus, E19
8,103,295plk-1TAMissense M > K, E12
8,127,908Intergenic*T insertion12
8,128,979Intergenic*C insertion30
8,154,518IntergenicTA10
  • a Chromosome III position from the WS200 reference version of Wormbase.

  • b The wild-type sequence.

  • c The changes found in the or683 genome. For insertions, the alteration takes place immediately following the indicated position.

  • d For mutations that cause protein coding changes, we have classified the loci as NE for not essential and E for essential.

  • e The total number of Illumina reads for the position. Only mutations with ≥10 reads were considered and furthermore, >90% of the reads had to show the alteration presented in column 4.

  • f These apparent mutations may represent the wild-type sequence in some backgrounds because expressed sequence tags available from WormBase also show these alterations.