Table 1  Mutations present in the or600sd,ts interval
PositionaGeneWTbAlterationcNotesdReadse
3,645,769unc-93CAIntron20
3,645,821unc-93TGIntron58
3,716,562C14B1.6*C insertionIntron22
3,805,251toh-1CSingle base deletionIntron591
3,830,219Intergenic*G insertion23
3,885,443tir-1*G insertionIntron30
3,971,283msi-1TAIntron45
3,997,832IntergenicAT142
4,007,014pdx-1*C insertionIntron22
4,017,108tbb-2CTMissense G > E, E614
4,019,053Intergenic*C insertion920
4,019,157Intergenic*G insertion1104
4,032,835hum-5*C insertionIntron72
4,056,056rcq-5ATIntron28
4,056,057rcq-5TAIntron28
4,056,058rcq-5ACIntron29
4,059,721his-70TSingle base deletionAltered C terminus, NE112
4,074,141unc-79TSingle base deletionIntron341
4,091,848C03C10.2*C insertionIntron122
4,209,222IntergenicCA1959
4,209,223IntergenicAC2105
4,209,244IntergenicCA2694
4,209,245IntergenicGC2546
4,210,819F43C1.1*G insertionIntron716
4,286,570R10E4.3CTIntron30
4,445,015Intergenic*G insertion24
4,447,068ceh-43*G insertionIntron81
4,447,641ceh-43*C insertionIntron83
4,502,805cct-5*G insertionIntron1140
4,530,454cdh-4ASingle base deletionIntron55
4,605,001sel-2TSingle base deletionIntron25
4,612,783Intergenic*C insertion411
4,640,496clec-154*G insertionAltered C terminus, NE195
4,684,393T04A8.3GSingle base deletionPseudogene45
4,687,673Intergenic*C insertion82
4,689,556T04A8.5*C insertionIntron64
4,708,949Intergenic*C insertion780
4,712,953T04A8.15CGfAnnotation error?626
4,738,437ras-2CTMissense G > R, NE53
4,808,804Intergenic*C insertion91
4,947,903Intergenic*C insertion59
4,962,073C27F2.5.1TSingle base deletionIntron44
5,105,425F54D8.6*C insertionIntron280
5,105,939F54D8.6*G insertionfAnnotation error?133
5,333,260F52C9.1*C insertionfAnnotation error?977
  • a Chromosome III position from the WS200 reference version of WormBase.

  • b The wild-type sequence.

  • c The changes found in the or600 genome. For insertions, the alteration takes place immediately following the indicated position.

  • d For mutations that cause protein coding changes, we have classified the loci as NE for not essential and E for essential.

  • e The total number of Illumina reads for the position. Only mutations with ≥20 reads were considered and furthermore, >75% of the reads had to show the alteration presented in column 4.

  • f These apparent mutations may represent the wild-type sequence in some backgrounds because expressed sequence tags also show these alterations.