Table 3  Analysis of incidental crossovers
Gfp*+ spore classNdeI digest (site1/site2)No. of spore clones (% of class)Configuration of markers is consistent with:
SPO11+spo11-HA
Rfp CfpCut/cut67/72 (93.0)53/53 (100.0)Single crossover associated with GFP* conversion
Cut/uncut2/72 (2.8)0/53Noncrossover conversion of gfp*-atg with distal incidental exchange
Uncut/cut3/72 (4.2)0/53Noncrossover conversion of gfp*-R215X with proximal incidental exchange
Rfp+ Cfp+Uncut/uncut2/2 (100.0)8/9 (88.9)Single crossover associated with GFP* conversion
Cut/uncut0/21/9 (11.1)Noncrossover conversion of gfp*-atg with proximal incidental exchange
Rfp+ CfpUncut/cut43/46 (93.5)42/45 (93.3)Noncrossover conversion of gfp*-R215X
Cut/cut3/46 (6.5)3/45 (6.7)Crossover associated with GFP* conversion with proximal incidental exchange
Rfp Cfp+Cut/uncut9/9 (100.0)20/23 (87.0)Noncrossover conversion of gfp*-atg
Cut/cut0/92/23 (8.7)Crossover associated with GFP* conversion with distal incidental exchange
Uncut/uncut0/91/23 (4.3)Crossover associated with GFP* conversion with proximal incidental exchange
  • Single Gfp*+ spores of the four possible flanking marker configurations were sorted, allowed to grow, and then genotyped for the NdeI sites diagrammed in Figure 5A. Data were pooled from two independent cultures (≥39 spores analyzed per culture). The agreement with expected marker patterns is described for each class.