TABLE 1

Summary of results of study

ConstructPromoter (extent)a3′-UTRDesignGFP loss (counts)/effective cutting ratedHoming fractione (counts)ExpressionfReferences
alyaly (−875:+97)Hsp70AbHEG-138% (417/1094) 49 ± 2%2 ± 1% (2/94)SCWang and Mann (2003)
β-Tub85Dβ-Tub85D (−188:+230)Hsp70AbHEG-170% (688/985) 90 ± 2%2 ± 1% (1/94)SC, SPMichiels et al. (1989)
Mst87FMst87F (−551:+240)Hsp70AbHEG-161% (638/1041) 79 ± 2%1 ± 1% (0/94)SPKuhn et al. (1988)
Hsp26Hsp26 (−499:+237)Hsp70AbHEG-150% (162/322) 63 ± 4%11 ± 3% (12/94)SCGlaser et al. (1986); Glaser and Lis (1990)
Hsp70Hsp70bHsp70AbHEG-126% (78/296) 28 ± 3%74 ± 3% (225/287)HS inductionRong and Golic (2003)
Act5C-P (males)Act5C/P-intronHsp70AbHEG-16% (44/793) 7 ± 1%29 ± 6% (15/44)GermlineLaski et al. (1986); Bond-Matthews and Davidson (1988); Laski and Rubin (1989)
vasavasa (−234:)cβ-Tub56DHEG-233% (1234/3764) 38 ± 2%36 ± 1% (523/1234)GermlineHay et al. (1988); Sano et al. (2002)
bambam (−169:+186)β-Tub56DHEG-29% (357/3910) 10 ± 1%63 ± 3% (245/357)SGGonczy et al. (1997); Chen and Mckearin (2003)
Act5C-P (males)Act5C/P-intronβ-Tub56DHEG-253% (671/1272) 62 ± 2%32 ± 2% (252/671)GermlineLaski et al. (1986); Bond-Matthews and Davidson (1988); Laski and Rubin (1989)
Act5C-P (females)Act5C/P-intronβ-Tub56DHEG-235% (937/2685) 39 ± 1%50 ± 2% (526/937)GermlineLaski et al. (1986); Bond-Matthews and Davidson (1988); Laski and Rubin (1989)
  • The combined data from our experiments are summarized here. Estimates of the effective cutting rate and homing fraction after accounting for NHEJ events were obtained by MCMC using the mcmc package (C. J. Geyer) written in R (R Development Core Team 2010).

  • a All extents are measured with the transcriptional initiation site in FlyBase as +1.

  • b As used in 70I-ISceI in Rong and Golic (2006). Heat-shock induction was applied.

  • c Complete spliced 5′-UTR included.

  • d Fraction of target chromosomes cut (excluding sister chromatid repair events), corrected for NHEJ-o events. Standard deviation of the estimate is also provided.

  • a Fraction of repaired chromosomes exhibiting a homed insert. Standard deviation of the estimate is also provided.

  • f SC, spermatocyte; SG, spermatogonia; SP, spermatid.