TABLE 1

DMPP resistance of daf-2 alleles

AlleleClassaDaf-cbDMPP resistance (N)cAmino acid changedMutation location (domain)d
+01 ± 1 (405)
e136810.3 ± 0.34 ± 3 (210)S573LExtracellular (L2)
e137110.1 ± 0.32 ± 1 (254)G803EExtracellular (FnIII2)
m41114 ± 480 ± 9 (333)G383EExtracellular (Cys-rich)
m577109 ± 5 (351)C1045YExtracellular (FnIII2)
m59610.4 ± 0.490 ± 3 (235)G547SExtracellular (L2)
e137020.5 ± 0.799 ± 8 (156)P1465SIntracellular (kinase)
e1391266 ± 792 ± 4 (182)P1434LIntracellular (kinase)
e979298 ± 0.872 ± 6 (174)C146YExtracellular (L1)
  • a Classes are defined in Gems et al. (1998). Class 1 mutants are Daf-c, increased adult longevity (Age), and intrinsic thermotolerance (Itt) and exhibit low levels of L1 arrest at 25°. Class 2 mutants exhibit these defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. Class 1 and class 2 alleles also differ in their genetic interactions with daf-12 loss-of-function alleles.

  • b Dauer formation at 20° as reported in Gems et al. (1998), for comparison with DMPP resistance.

  • c Percentage survival on 0.75 mm DMPP at 20° (average ± SEM, n ≥ 3 independent experiments).

  • d Adapted from Patel et al. (2008).