TABLE 1

New GAL4, LexA, Split GAL4, and GAL80 vector backbones used in this study

Name5′-UTR IVSTransgene3′-UTR WPRETerminator
pBPGAL4.1UwGAL4ahsp70
pBPGAL4.2UwGAL4ahsp70
pBPGAL4.2Uw-2GAL4aSV40
pBPGAL4dUwGAL4 deletion variant II-9 (GADd)hsp70
pBPGAL4d.2UwGAL4 deletion variant II-9a (GADd)hsp70
pBPGAL4.2∷VP16UwGAL4∷VP16ahsp70
pBPGAL4.2∷p65UwGAL4∷p65ahsp70
pBPLexA∷GADdUwLexA∷GADdahsp70
pBPLexA∷GADflUwLexA∷GADflahsp70
pBPnlsLexA∷GADflUwnlsLexA∷GADflahsp70
pBPLexA∷VP16UwLexA∷VP16hsp70
pBPLexA∷p65UwLexA∷p65ahsp70
pBPnlsLexA∷p65UwnlsLexA∷p65ahsp70
pBPZpGAL4DBDUwZip-GAL4DBDahsp70
pBPp65ADZpUwp65AD-Zipahsp70
pBPGAL80Uw-1GAL80ahsp70
pBPGAL80Uw-2GAL80a+hsp70
pBPGAL80Uw-3+GAL80ahsp70
pBPGAL80Uw-4+GAL80a+hsp70
pBPGAL80Uw-5GAL80aSV40
pBPGAL80Uw-6+GAL80a+SV40
  • BP plasmid vector backbones are derived from pBPGUw (Pfeiffer et al. 2008) and contain the pUC19-derived bacterial origin of replication and ampicillin resistance gene, the PhiC31 attB site, the mini-white marker for identification of transformants in Drosophila, and the DSCP basal promoter. For more details see Figure 1 and Addgene plasmid 17575. Abbreviations: U, DSCP basal promoter; w, mini-white marker; nls, nuclear localization signal; IVS, intervening sequence within the 5′-UTR; WPRE, a woodchuck hepatitis virus post-transcriptional regulatory element within the 3′-UTR; TERMINATOR, the transcriptional terminator; and pBP, plasmid BP backbone.

  • a Drosophila codon-optimized transgene.