TABLE 1

Yeast strains used in this study

Strain nameGenotypeSource
Figure 1Aa
JDY88MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 sir2∷KanMX6 [CEN TRP1 HTA1-HTB1]This work
JDY230MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1]This work
JDY231MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R95A]This work
JDY232MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R95K]This work
JDY233MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R102A]This work
JDY234MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R102K]This work
Figure 1Bb
JDY235MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HTA1-HTB1]This work
JDY236MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HTA1-HTB1-R95A]This work
JDY237MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HTA1-HTB1-R95K]This work
JDY238MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HTA1-HTB1-R102A]This work
JDY239MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HTA1-HTB1-R102K]This work
JDY240MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HHT2-HHF2]This work
JDY241MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HHT2-HHF2-K16A]This work
JDY242MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HHT2-A75V-HHF2]This work
JDY243MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HHT2-K79R-HHF2]This work
JDY244MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR [CEN TRP1 HHT2-HHF2-R45C]This work
Figure 1Cc
JDY89MATa his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 sir2∷KanMX6 [CEN TRP1 HTA2-HTB2]This work
JDY245MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1]This work
JDY246MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R95A]This work
JDY247MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R95K]This work
JDY248MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R102A]This work
JDY249MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R102K]This work
Figure 1Dd
BY4742MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0
BY4741MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
JDY89See Figure 1C
JDY245See Figure 1C
JDY246See Figure 1C
JDY247See Figure 1C
JDY248See Figure 1C
JDY249See Figure 1C
Figure 2A
JDY89See Figure 1D
JDY245See Figure 1C
JDY246See Figure 1C
JDY247See Figure 1C
JDY248See Figure 1C
JDY249See Figure 1C
JDY250MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN LEU2 HTA1-HTB1-R95A,R102A]
Figure 2B
JDY251MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1] [pRS425 2μ LEU2]This work
JDY252MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [pJD84 CEN TRP1 HTA1-HTB1] [2μ LEU2 SIR2]This work
JDY253MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [pJD107 CEN TRP1 HTA1-HTB1-R95A] [pRS425 2μ LEU2]This work
JDY254MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [pJD107 CEN TRP1 HTA1-HTB1-R95A] [2μ LEU2 SIR2]This work
JDY255MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [pJD109 CEN TRP1 HTA1-HTB1-R102A] [pRS425 2μ LEU2]This work
JDY256MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [pJD109 CEN TRP1 HTA1-HTB1-R102A] [2μ LEU2 SIR2]This work
Figure 2C
JD257MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1] [pRS425 2μ LEU2]This work
JD258MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95A] [pRS425 2μ LEU2]This work
JD259MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95K] [pRS425 2μ LEU2]This work
JD260MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102A] [pRS425 2μ LEU2]This work
JD261MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102K] [pRS425 2μ LEU2]This work
JD262MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1] [2μ LEU2 SIR2]This work
JD263MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95A] [2μ LEU2 SIR2]This work
JD264MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95K] [2μ LEU2 SIR2]This work
JD265MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102A] [2μ LEU2 SIR2]This work
JD266MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102K] [2μ LEU2 SIR2]This work
Figure 2D
JDY245See Figure 1C
JDY246See Figure 1C
JDY248See Figure 1C
JDY250See Figure 2A
Figure 3A
JD267MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1] [CEN LEU2 SIR3]This work
JD268MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95A] [CEN LEU2 SIR3]This work
JD269MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95K] [CEN LEU2 SIR3]This work
JD270MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102A] [CEN LEU2 SIR3]This work
JD271MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102K] [CEN LEU2 SIR3]This work
JD272MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1] [CEN LEU2 SIR3-D205N]This work
JD273MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95A] [CEN LEU2 SIR3-D205N]This work
JD274MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95K] [CEN LEU2 SIR3-D205N]This work
JD275MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102A] [CEN LEU2 SIR3-D205N]This work
JD276MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102K] [CEN LEU2 SIR3-D205N]This work
Figure 3B
JD277MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1] [CEN LEU2 SIR3]This work
JD278MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95A] [CEN LEU2 SIR3]This work
JD279MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95K] [CEN LEU2 SIR3]This work
JD280MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102A] [CEN LEU2 SIR3]This work
JD281MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102K] [CEN LEU2 SIR3]This work
JD282MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1] [CEN LEU2 SIR3-D205N]This work
JD283MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95A] [CEN LEU2 SIR3-D205N]This work
JD284MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95K] [CEN LEU2 SIR3-D205N]This work
JD285MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102A] [CEN LEU2 SIR3-D205N]This work
JD286MATα his3Δ200 leu2Δ1 trp1Δ63 lys2Δ0 ura3-167 met15Δ0 ade2∷his RDN1∷mURA3/HIS3 RDN1∷Ty1-MET15 TELV∷ADE2 hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R102K] [CEN LEU2 SIR3-D205N]This work
Figure 3C
JD277See Figure 3B
JD278See Figure 3B
JD279See Figure 3B
JD280See Figure 3B
JD281See Figure 3B
JD282See Figure 3B
JD283See Figure 3B
JD284See Figure 3B
JD285See Figure 3B
JD286See Figure 3B
Figure 4
JDY245See Figure 1C
JDY246See Figure 1C
JDY248See Figure 1C
Figure 5A
JDY230See Figure 1A
JDY231See Figure 1A
JDY232See Figure 1A
JDY233See Figure 1A
JDY234See Figure 1A
JDY287MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 ppr1∷HIS3 adh4∷URA3-TEL-VIIL ADE2-TEL-VR hta2-htb2∷HygMX4 hta1-htb1∷NatMX4 [CEN TRP1 HTA1-HTB1-R95A,R102A]
Figure 5B
JDY245See Figure 1C
JDY246See Figure 1C
JDY247See Figure 1C
JDY248See Figure 1C
JDY249See Figure 1C
JDY250See Figure 2B
Figure 5C
JDY245See Figure 1C
JDY246See Figure 1C
JDY247See Figure 1C
JDY248See Figure 1C
JDY249See Figure 1C
JDY250See Figure 2B
Figure 6A
BY4741 sir2∷KanMX4
BY4741 fob1∷KanMX4
JDY289MATα his3Δ200 leu2Δ0 lys2Δ0 trp1Δ63 ura3Δ0 met15Δ0 hta2-htb2∷HygMX4 hta1-htb1∷G418 [CEN LEU2 HTA1-HTB1]This work
JDY290MATα his3Δ200 leu2Δ0 lys2Δ0 trp1Δ63 ura3Δ0 met15Δ0 hta2-htb2∷HygMX4 hta1-htb1∷G418 [CEN LEU2 HTA1-HTB1-R95A]This work
JDY291MATα his3Δ200 leu2Δ0 lys2Δ0 trp1Δ63 ura3Δ0 met15Δ0 hta2-htb2∷HygMX4 hta1-htb1∷G418 [CEN LEU2 HTA1-HTB1-R102A]This work
JDY292MATα his3Δ200 leu2Δ0 lys2Δ0 trp1Δ63 ura3Δ0 met15Δ0 hta2-htb2∷HygMX4 hta1-htb1∷G418 [CEN LEU2 HTA1-HTB1-R95A,R102A]This work
JDY296MATα his3Δ200 leu2Δ0 lys2Δ0 trp1Δ63 ura3Δ0 met15Δ0 hta2-htb2∷HygMX4 hta1-htb1∷G418 [CEN LEU2 HTA1-HTB1-R95K]This work
JDY297MATα his3Δ200 leu2Δ0 lys2Δ0 trp1Δ63 ura3Δ0 met15Δ0 hta2-htb2∷HygMX4 hta1-htb1∷G418 [CEN LEU2 HTA1-HTB1-R102K]This work
Figure 6Bd
BY4741 sir2∷KanMX4
JDY289See Figure 6A
JDY290See Figure 6A
JDY291See Figure 6A
JDY292See Figure 6A
  • a All these strains were derived from UCC3505 and were generated by PCR-mediated gene replacement.

  • b These strains are UCC3505 transformed with an extra plasmid-borne copy of the indicated histones in addition to the two endogenous gene copies.

  • c All these strains are in the GRF167 background and were generated by PCR-mediated gene replacement.

  • d All these strains are in the BY background.