TABLE 1

Comparison of experimental design between the crosses

Experimental designCross ICross II
Number of mice
    TissueSex
    AdiposeFemale147136
Male143133
    BrainFemale133140
Male118149
    LiverFemale149141
Male142156
    MuscleFemale158123
Male139148
Diets
    Purina Chow containing 4% fat (Ralston Purina, St. Louis)8 wk8 wk
    Western diet 88137 containing 42% fat and 0.15% cholesterol (Harlan, Teklad, Madison, WI)16 wk12 wk
Duration of fasting4 hr12 hr
Age of mice when killed24 wk20 wk
Genotyping platform/density of SNPsParAllele 1100 SNP array ± 300 SNPsParAllele 2000 SNP array (n = 150); Illumina 1536 SNP array (n = 150)
Number of SNPs in study1,3261,326
Expression array platformsAgilent Mouse TOE75K Array1Agilent Mouse TOE75K Array1
Number of transcripts
    Adipose23,56823,560
    Brain23,56823,563
    Liver23,56823,558
    Muscle23,45823,458