TABLE 2

Expression of GFP from non-AUG reporters in iftb-1(nb101) mutants

LineReporterwtiftb-1(nb101)/+iftb-1(nb101)
1AUG+++++++++++++++
2GUG+++NA
3CUG++++
4UUG+++++
5AAG
6ACG+++
7AGGNA
8AUA+++
9AUC+++/−
10AUU++++++
11CCGNA
12GUC
13UUC
14GGCNA
15UACNA
  • The GFP expression intensity was scored under a dissecting microscope as follows: −, no expression; +, weak expression; ++, moderate expression; +++, high expression. The GFP expression from reporters carrying the native AUG start codon (line 1) is considerably higher (+++++) than that from any other non-AUG reporters. NA, strain not available for scoring. wt, wild type.