TABLE 1

Strains used in this study

StrainGenotypeSource
LRY1007 (W303)MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1R. Rothstein
LRY0800W303 MATα sir2Δ∷TRP1 LEU2∷sir2-N345A
LRY0804W303 MATα LEU2∷sir2-N345A
LRY1021MATa his4P. Schatz
LRY1068W303 MATα sir2Δ∷TRP1
LRY1815W303 MATα hmr-ΔI LEU2∷sir2-N345A
LRY2148W303 MATα TELVIR∷STUFFER
LRY2150W303 MATα TELVIR∷HMR-E
LRY2302W303 MATα HMR-ΔtRNAThr
LRY2303W303 MATα HMR-ΔtRNAThr LEU2∷sir2-N345A
LRY2309W303 MATα hmrI ΔtRNAThr
LRY2352W303 MATα hmrI ΔtRNAThr LEU2∷sir2-N345A
LRY2315W303 MATα hmrI
LRY2316W303 MATα hmrI LEU2∷sir2-N345A
LRY2379W303 MATα [pJR831; PGAL1-HO; URA3]a
LRY2384W303 MATα hmr-ΔI [pJR831; PGAL1-HO; URA3]a
LRY2467W303 MATa [pJR831; PGAL1-HO; URA3]a
LRY2482W303 MATα sir4ΔHIS3 [pJR831; PGAL1-HO; URA3]a
YAB65W303 MATα hmr-ΔE (546–30; 516-bp deletion)A. Brand
YAB71W303 MATα hmr-ΔE (358–223; 135-bp deletion)A. Brand
  • a Brackets denote transformation with the indicated plasmid. See materials and methods for plasmid details.