TABLE 1

Strains used in this study

StrainGenotypeSource or reference
A28pabaA6 biA1FGSC (accession no. A28)
GR5pyrG89 wA3 pyroA4FGSC (accession no. A773)
TNO2A3pyrG89; argB2; pyroA4 nkuA∷argB
AHS2pyrG89; argB2; Δbud3∷pry-4 pyroA4 nkuA∷argBThis study
AHS3pyrG89; argB2; Δbud3∷pyroA pyroA4 nkuA∷argBThis study
AHS5pyrG89; argB2; Δrho4∷pyroA pyroA4 nkuA∷argBThis study
AHS7pyrG89; argB2; Δmsb1∷pyroA pyroA4 nkuA∷argBThis study
AHS25pyrG89; argB2; Δrho4∷pyroA; Δbud3∷pyrG; pyroA4 nkuA∷argBThis study
AHS30sepA∷gfp∷pyr-4; pyrG89 pabaA1; Δbud3∷pyroA; yA2This study
AHS252yA2; argB2; pyroA4This study
AAV123.1pyrG89 sepA∷gfp∷pyr-4; argB2; pyroA4 ΔnkuA∷argBVirag et al. (2007)
ASH630sepA1; pyrG89; wA3Lab stock
ACP115tpmA∷GFP∷pyr-4; pyrG89; wA3Pearson et al. (2004)
AKS70sepA∷gfp∷pyr-4; pyrG89 pabaA1; yA2Sharpless and Harris (2002)
AHS41pyrG89; argB2; gfp∷bud3∷pyr4; pyroA4; nkuA∷argBThis study
AHS43pyrG89;argB2;alcA∷gfp∷rho4; pyr-4; nkuA∷argBThis study
AHS51pyrG89; argB2;gfp∷bud3∷pyr4; pyroA4; sepA1 nkuA∷argBThis study
AHS53sepA1; tpmA∷GFP∷pyr4;pyrG89This study
AHS3C2Δbud3 suppressorThis study
AJM34sepH 1; pabaA6; lysB5; chaA1
AHS61pyrG89; sepH 1This study
AHS62pyrG89; gfp∷bud3∷pyr4; sepH 1This study