fem-3(lf) X0 germ lines sense a single pair of asynapsed autosomes but not the unpaired X chromosome
| Asynapsed chromosomes | No. apoptotic bodies/gonads | ||
|---|---|---|---|
| RNAi | N2 XX | fem-3(lf) X0 | |
| L4440 | NA | 5.6 ± 0.3 | 1.8 ± 0.2 |
| zim-2 | V | 8.2 ± 0.6* | 4.9 ± 0.6* |
| zim-1 | II and III | 12.0 ± 1.5* | 3.9 ± 0.5* |
| syp-1 | All | 14.0 ± 0.8* | 5.2 ± 0.5* |
| chk-1 | NA | 5.1 ± 0.8 | 1.8 ± 0.7 |
| syp-1;chk-1 | All | 4.6 ± 0.8 | 2.8 ± 0.7 |
| pch-2 | NA | 7.5 ± 1.0 | 1.5 ± 0.3 |
| syp-1;pch-2 | All | 9.1 ± 1.9* | 2.4 ± 0.4 |
| him-8 | X | 9.9 ± 1.1* | 2.4 ± 0.3 |
Number of apoptotic nuclei/gonad arm was determined by CED-1-GFP 48 hr post-L4. Minimum of 15 gonad arms were scored for each genotype. Average number of bivalents/univalents in diakinesis oocytes by DAPI staining for L4440 = 5.7, zim-2(RNAi) = 6.5, fem-3(e1996); zim-2(RNAi) = 6.4, zim-1(RNAi) = 7.0, fem-3(e1996); zim-1(RNAi) = 7.4, and him-8(RNAi) = 6.4. syp-1(RNAi) efficiency was determined by monitoring plates for dead embryos and male progeny. The data shown are means ± SEM Statistical comparisons between the mutants and L4440 were conducted using the two-tailed Mann-Whitney test. *P < 0.001.