TABLE 1

fem-3(lf) X0 germ lines sense a single pair of asynapsed autosomes but not the unpaired X chromosome

Asynapsed chromosomesNo. apoptotic bodies/gonads
RNAiN2 XXfem-3(lf) X0
L4440NA5.6 ± 0.31.8 ± 0.2
zim-2V8.2 ± 0.6*4.9 ± 0.6*
zim-1II and III12.0 ± 1.5*3.9 ± 0.5*
syp-1All14.0 ± 0.8*5.2 ± 0.5*
chk-1NA5.1 ± 0.81.8 ± 0.7
syp-1;chk-1All4.6 ± 0.82.8 ± 0.7
pch-2NA7.5 ± 1.01.5 ± 0.3
syp-1;pch-2All9.1 ± 1.9*2.4 ± 0.4
him-8X9.9 ± 1.1*2.4 ± 0.3
  • Number of apoptotic nuclei/gonad arm was determined by CED-1-GFP 48 hr post-L4. Minimum of 15 gonad arms were scored for each genotype. Average number of bivalents/univalents in diakinesis oocytes by DAPI staining for L4440 = 5.7, zim-2(RNAi) = 6.5, fem-3(e1996); zim-2(RNAi) = 6.4, zim-1(RNAi) = 7.0, fem-3(e1996); zim-1(RNAi) = 7.4, and him-8(RNAi) = 6.4. syp-1(RNAi) efficiency was determined by monitoring plates for dead embryos and male progeny. The data shown are means ± SEM Statistical comparisons between the mutants and L4440 were conducted using the two-tailed Mann-Whitney test. *P < 0.001.