Summary results for generated Tol2 mutant mouse lines

Seed male × FVB femalesTotal no. of pupsNo. of GFP+ pupsGenes mutatedPercentage of GFP+ pups
Litter 174Spsb1; Frem157
Litter 273Ptprd; 4930473A06Rik43
Litter 3102ND20
Litter 472Genscan; ENSMUST0000010724229
Litter 574Genscan/Geneid; Genscan; Ptprd57
Litter 672Genscan29
Litter 774Genscan57
Litter 892ND22
Litter 972061008F07Rik; Geneid29
Litter 1062Ptprd; Myo3b33
Litter 1193Geneid/Genscan; Inpp5b33
Litter 12103Geneid/Genscan; Genscan; Genscan30
Litter 13107Geneid/Genscan; Genscan70
Litter 1482Nfia25
Litter 15103Genscan30
Total12145Average 38
  • ND, not determined due to technical limitations. Genscan, gene predictions based on transcriptional, translational, and donor/acceptor splicing signals, as well as the length and compositional distributions of exons, introns, and intergenic regions. Geneid is a program that predicts genes in anonymous genomic sequences designed with a hierarchical structure. In the first step, splice sites, start and stop codons are predicted and scored along the sequence using position weight arrays. Next, exons are built from the sites. Exons are then scored as the sum of the scores of the defining sites, plus the log-likelihood ratio of a Markov model for coding DNA. Finally, from the set of predicted exons, the gene structure is assembled, maximizing the sum of the scores of the assembled exons. Known genes or transcripts (Riken cDNAs or ESTs) are shown in italics. Genscan/Geneid, indicates transposon insertion in a locus with both predicted Genscan and Geneid.