TABLE 4

cosB suppressors do not have trans effects, and Nu1 suppressors do not have cis effects

LineHelperaProphageHelper yieldbProphage yieldb
1λ-P1 Nu1+cosBλ Nu1hy1E20G3.22 (0.74)0.49 (0.21)
2λ-P1Nu1hy2cosBλ Nu1hy1E20G1.10 (0.21)4.55 (1.2) × 10−5
3λ-P1Nu1hy1 cosBλC59GcosBλ2.55 (0.43)0.51 (0.12)
4λ-P1Nu1hy1 cosBλC59GcosB211.79 (0.39)2.14 (0.44)
5λ-P1Nu1hy1 cosBλA117CcosBλ1.25 (0.33)0.31 (0.06)
6λ-P1Nu1hy1 cosBλA117CcosB211.6 (0.35)2.75 (0.28)
  • The genetic background for helper phages was λ-P1:5R cI857 nin5 Δbet-gam∷kan. Tandem double prophages were constructed by lysogenizing MF532 as described in materials and methods.

  • a The genetic background for helper phages was λ-P1:5R cI857 nin5 Δbet-gam∷kan.

  • b Values in parentheses are the standard error of the mean (3 ≤ n ≤ 5).