TABLE 1

Masculinization of flies with a female genotype caused by Mdtra RNAi

Injected substanceNo. of injected embryosTotal no. of hatched adultsFemale genotypeMale genotype
StrainNo.External sexual phenotypeNo.External sexual phenotype
F+aRinger2733246107Female139Male
Mdtra dsRNA20702340Female0Female
105Intersexualc0Intersexual
4Male134Male
FDbRinger9504424Female20Male
Mdtra dsRNA322718989Femaled0Female
2Intersexualc0Intersexual
1Male97Male
  • a Autosomal M strain: Female genotype is XX; bwb pw/bwb pw; F+/F+ (phenotype bwb pw), and male genotype is XX; M pw+ bwb+/pw bwb; F+/F+ (phenotype bwb+ pw+).

  • b Strain FD/Fman: Female genotype is XX; FD Ba/Fman Ba+ (phenotype Ba), and male genotype is XX; Fman Ba+/Fman Ba+ (phenotype Ba+).

  • c Flies with mixed external sexually dimorphic structures, e.g., with male genitalia but female eye distance, or flies with mixed external genitalia and male or female eye distances.

  • d All with ovotestes.