TABLE 2

Mutant terminases with broad packaging specificity

LineHelperPassive prophageProphage yielda (SEM)bPassive prophageProphage yielda (SEM)bDiscrimination indexc
1λ-P1 Nu1+λ1.02 (0.36)213.24 (1.37) × 10−53.1 × 104
2λ-P1 Nu1hy51 (H21 T21 H21 w21)λ8.02 (0.9) × 10−5212.62 (0.29)3.3 × 104
3λ-P1 Nu1hy2 (H21 T21 H21 wλ)λ2.9 (0.55) × 10−6212.15 (0.175)7.4 × 105
4λ-P1 Nu1hy1 (Hλ Tλ H21 wλ)λ0.27 (0.040)212.86 (0.40)1.1 × 101
5λ-P1 Nu1hy1E20Dλ0.36 (0.14)210.41 (0.17)1.1
6λ-P1 Nu1hy1E20Gλ1.03 (0.14)211.90 (0.35)1.8
7λ-P1 Nu1hy1Q23Rλ0.70 (0.14)211.12 (0.22)1.6
8λ-P1 Nu1hy1Q23Kλ1.35 (0.18)211.29 (0.21)0.95
9λ-P1 Nu1hy1L40Iλ0.90 (0.20)212.55 (0.17)2.8
10λ-P1 Nu1hy3 (H21 Tλ H21 wλ)λ0.03 (0.006)211.63 (0.24)54
11λ-P1 Nu1hy4 (Hλ T21 H21 wλ)λ0.15 (0.04)211.72 (0.33)11
  • a Plaque-forming units/induced lysogen.

  • b Values in parentheses are the standard error of the mean (3 ≤ n ≤ 5).

  • c Discrimination index is the yield of the cognate passive prophage/yield of the noncognate passive prophage.