TABLE 1

GFP∷PGL-1 screen positives

Gene nameWormbase IDGene class codecGFP∷PGL-1 in the P0 germ lineGFP∷PGL-1 in F1 embryosPGL-1 phenotype preceded by general embryo defects?Endogenous PGL-1
DescriptionAltered levelAltered patternAltered leveldAltered patternStainedSame as GFP
adr-1H15N14.1RNA adenosine deaminaseAbnormalYes
air-1K07C11.2Aurora kinaseCLowDiffuseYes
apc-2K06H7.6Anaphase-promoting complex 2ULowDiffuseYes
arl-1F54C9.10GTP-binding ADP-ribosylation factorGDiffuseYes
atx-2D2045.1aAtaxin-related proteinLowDiffuseNoXX
bir-1T27F2.3Aurora B kinase complexCLowNoXX
cct-2T21B10.7Cytosolic “T complex” chaperoninTDiffuseNoXX
cct-3F54A3.3Cytosolic T complex chaperoninTLowDiffuseYes
cct-7T10B5.5Cytosolic T complex chaperoninTLowYes
cct-8Y55F3AR.3abCytosolic T complex chaperoninTLowDiffuseYesXX
cdc-25.1K06A5.7Cell division cycle 25.1 phosphataseCLowDiffuse. Puncta in multiple cellsNoXX
cdc-42R07G3.1Cell division cycle 42 Rho GTPaseCLowDiffuseNoX
cdk-1T05G5.3Cyclin-dependent kinase 1CLowDiffuseYes
cdk-9H25P06.2Cyclin-dependent kinase 9CLow or und.YesXX
ceh-21T26C11.6ONECUT class homeobox proteinLowNoX
cgh-1C07H6.5Germ-line DEAD-box RNA helicaseLowNoXX
cpf-2F56A8.6Cleavage and polyadenylation factorSLowDiffuse in P cellsNoX
cpl-1T03E6.7Cathepsin L-like cysteine protease 1LowDiffuseYes
csr-1F20D12.1Secondary RNA slicing ArgonauteHighLarge granulesHighNoXX
cyb-3T06E6.2Cyclin B3 proteinLowDiffuseYes
cyk-4K08E3.6Rho GTPase-activating protein (GAP)CUnd.Yes
cyp-31A3Y17G9B.3CYP7B1 homolog —vitamin D deficiencyLowAbnormalNoXX
dhc-1T21E12.4Dynein heavy chainCDiffuseYes
dlc-1T26A5.9Dynein light chainNLowYes
dnc-1ZK593.5Dynactin/GLUED orthologsCLowYesXX
dom-6C09G4.3Cks/Suc1 ortholog. promotes cyclin B degradationCLowDiffuseYesXX
dpf-1T23F1.7Prolyl oligopeptidaseLowDiffuseNoX
dyci-1C17H12.1Dynein cytoplasmic intermediate chainDiffuseYes
eft-1ZK328.2Tu domain containing elongation factorHighDiffuseHigh in P cellsDiffuse in somaNoXX
egg-3F44F4.2Protein–tyrosine phosphataseCLowDiffuseYes
egl-45C27D11.1Eukaryotic translation initiation factor 3 subunit 10RLow or und.NoXX
eif-3.FD2013.7Eukaryotic translation initiation factor 3 subunit 5RLowNoXX
elc-1Y82E9BR.15Elongin CUUndetectableUnd.YesXX
emb-9K04H4.1CollagenLowNoX
fbxa-81T24C2.4F-box domainULowNoX
fbxb-51K05F6.3F-box domainUHighHigh in P cellsLarge granulesNoXX
fbxb-60F55C9.7F-box domainUHighNoX
flp-13F33D4.3FMRF-like amide neurotransmitterHighHigh in P cellsDiffuse in somaNoX
glb-27W01C9.5UnknownLowNoX
gon-1F25H8.3Secreted metalloprotease (MPT)LowDiffuseNoXX
gsk-3Y18D10A.5Glycogen synthase kinase required for polarization of EMS and P2 blastomeresCLowDiffuse in multiple cellsYes
his-40K03A1.1Similarity to histones (pseudogene?)Diffuse in P cellsYes
his-48B0035.8H2B histoneLowYes
hsp-6C37H5.8HSP70-like mitochondrial chaperoneLowNoX
ify-1C27A2.3Ligand of Fizzy protein. Securin?CDiffuseYes
imb-4ZK742.1Exportin-1/CRM1 nuclear exportinNDiffuse in P cellsNoXX
imb-5Y48G1A.5abCSE1/CAS-like nuclear importinNDiffuse in P cellsNoXX
inx-8ZK792.2Innexin/GAP junction componentLowNoX
let-70M7.1E2 ubiquitin-conjugating enzymeULowDiffuseYes
let-711F57B9.2NOT1 deadenylation componentHighDiffuseHighNoXX
let-92F38H4.9Catalytic subunit of PP2A phosphataseDiffuseYesXX
let-99K08E7.3DEP domain. Spindle orientationCLowNoXX
lin-41C12C8.3RING finger B box coiled-coil proteinLow in someNoX
lmn-1DY3.2Nuclear type B laminNDiffuse in P cellsNoXX
mag-1R09B3.5Mago nashi. Exon–exon junction factorSLow in someNoX
mbk-2F49E11.1Minibrain DYRK2 kinaseCLowNoXX
mcm-2Y17G7B.5MCM licensing factor 2MLowDiffuse in P cellsYesXX
mcm-4Y39G10AR.14MCM licensing factor 4MLowDiffuse in P cells, puncta in somaYesXX
mcm-5R10E4.4MCM licensing factor 5MDiffuse in P cellsYesXX
mcm-6ZK632.1MCM licensing factor 6MDiffuse in P cells, high puncta in somaYesXX
mcm-7F32D1.10MCM licensing factor 7MDiffuse in P cellsYesXX
mdt-6Y57E12AL.5Transcriptioinal mediator Med6-likeLowDiffuseYes
mel-26ZK858.4Adaptor of the E3 ubiquitin ligaseUDiffuseYes
mix-1M106.1SMC2-like condensing complex factorLowNoX
nmy-2F20G4.3Nonmuscle myosin IICLowDiffuseLowDiffuseYesXX
npp-10ZK328.5NUP98-like nucleoporinNDiffuse in P cellsNoXX
npp-20Y77E11A.13NucleoporinNLowDiffuseYesXX
npp-6F56A3.3NUP160-like nucleoporinNDiffuse in P cellsNoXX
npp-7T19B4.2NucleoporinNDiffuse in P cellsNoXX
npp-9F59A2.1NucleoporinNDiffuse in P cellsNoXX
paa-1F48E8.5Regulatory subunit of PP2A phosphataseDiffuseYesXX
par-1H39E23.1Serine/threonine kinaseCLow or und.DiffuseNoXX
par-2F58B6.3C3HC4-type RING-finger proteinCLow or und.Yes
par-5M117.214-3-3-containing proteinCLowDiffuseNoXX
par-6T26E3.3PDZ-domain-containing proteinCLow or und.DiffuseYes
pas-4C36B1.4α4 20S proteasome core subunitPLow or und.YesXX
pas-5F25H2.9α5 20S proteasome core subunitPLow or und.Yes
pbs-1K08D12.1β1 20S proteasome core subunitPLowDiffuseYesXX
pbs-3Y38A8.2β3 20S proteasome core subunitPUnd. in someYes
pbs-4T20F5.2β4 20S proteasome core subunitPDiffuseYes
pbs-5K05C4.1β5 20S proteasome core subunitPDiffuseLowDiffuseYesXX
pbs-6C02F5.9β6 20S proteasome core subunitPDiffuseYes
pbs-7F39H11.5β7 20S proteasome core subunitPDiffuseYes
pgl-1ZK381.4RGG box motifs.Und.No
phi-10Y46G5A.4DEAD-box RNA helicase BRR2SHighHighNoXX
phi-12M03F8.3HAT repeat. mRNA splicingSLowNoXX
phi-21T05H4.6Translation release factorLowDiffuseNoXX
phi-6K02F2.3snRNP-associated factorSHighHigh in P cellsDiffuse in somaNoXX
phi-62F49C12.12UnknownLowNo?XX
phi-7D1081.8mRNA splicing protein CDC5 (Myb)SHighDiffuseHigh in P cellsNoXX
plk-1C14B9.4Serine/threonine polo-like kinaseCLowDiffuseYesXX
pri-1F58A4.4DNA Pol α-primase subunit DLowNoXX
prp-8C50C3.6U5 snRNA-associated splicing factorSHighDiffuseHighDiffuseNoXX
prs-1T20H4.3Proline tRNA ligaseLowNo?XX
puf-3Y45F10A.2PUF family RNA-binding proteinDiffuseNoXX
ran-1K01G5.4RAN-associated GTP-binding proteinNLowDiffuseNoXX
ran-4R05D11.3Nuclear transport factor 2 (NTF2)NLowDiffuse in P cellsNoXX
rnp-4R07E5.14Exon–exon junction complex factorSDiffuse in P cellsYes
rnr-1T23G5.1Ribonucleotide reductaseLow or und.Diffuse in P cellsNoXX
rnr-2C03C10.3Ribonucleotide reductaseDiffuse in P cellsNoXX
rpa-1F18A1.5Large subunit of replication protein ADiffuse in P cells, high puncta in somaYes
rpb-2C26E6.4RNA Pol II β-subunitDiffuse in P cellsYesXX
rpl-12JC8.3Ribosomal protein large subunit 12RLowNoX
rpl-18Y45F10D.12Ribosomal protein large subunit 18RLowYesXX
rpl-20E04A4.8Ribosomal protein large subunit 18aRLowNoXX
rpl-36F37C12.4Ribosomal protein large subunit 36RLowNoXX
rpn-1T22D1.9aProteasome regulatory particlePDiffuseYes
rpn-11K07D4.3Proteasome regulatory particlePDiffuse in P cellsYes
rpn-3C30C11.2Proteasome regulatory particlePDiffuseYes
rps-0B0393.1Ribosomal protein small subunit 2ARLowNoXX
rps-20Y105E8A.16Ribosomal protein small subunit 20RLowYes
rps-22F53A3.3Ribosomal protein small subunit 15aRLowNoXX
rps-24T07A9.11Ribosomal protein small subunit 24RLowNoX
rps-3C23G10.3aRibosomal protein small subunit 3RLowNoX
rps-5T05E11.1Ribosomal protein small subunit 5RLowNoX
rps-8F42C5.8bRibosomal protein small subunit 8RLowDiffuseYes
rps-9F40F8.10Ribosomal protein small subunit 9RLowNoXX
rpt-1C52E4.4Proteasome regulatory particlePLowDiffuseYes
rpt-3F23F12.6Proteasome regulatory particlePDiffuseYes
rpt-5F56H1.4Proteasome regulatory particlePDiffuseYes
rsp-7D2089.1SR protein (splicing factor) 7SLowDiffuse in P cellsNoXX
ruvb-1C27H6.2RuvB-like DNA helicaseLowNoX
ruvb-2T22D1.10RuvB-like DNA helicaseLowNoX
scp-1D2013.8WD repeat, sreb cleavage proteinGLow in someNoX
skp-1T27F2.1SKI-binding protein (SKIP) orthologsSHighHighDiffuse in somaNoXX
snr-2W08E3.1Core splicesome componentSLowYes
spd-5F56A3.4Dynein heavy chain 1CDiffuseYes
sqv-6Y50D4C.4XylosyltransferaseCLow or und.Yes
srh-81W03F9.7Serpentine receptor class HLowNoX
sri-25C41G6.10Serpentine receptor class ILowNoX
srj-6F31F4.8Serpentine receptor class JLowNoX
sru-41Y51H7BR.6Serpentine receptor class ULowNoX
srx-38C03A7.5Serpentine receptor class XLowNoX
tag-198F09G8.2Putative DNase IILowNoX
tag-49AC7.1G-protein receptorLowNoXX
tbb-2C36E8.5β-TubulinDiffuseYesXX
tbg-1F58A4.8γ-TubulinCLowYes
ttr-14T05A10.3Transthyretin-related family domainLowNoX
uba-1C47E12.5Ubiquitin UBA1/UBE1 relatedULowDiffuseYesXX
ubl-1H06I04.4Ribosomal S31 fused to ubiquitinULowDiffuseLowDiffuseNoXX
unc-112C47E8.7PH domain mitogen-inducible gene-2LowNoX
unc-32ZK637.8Vacuolar ATPase subunitVLowYes
vha-13Y49A3A.2Vacuolar ATPase subunitVLowYesXX
vha-19Y55H10A.1Vacuolar ATPase subunitVLowYes
vha-2R10E11.2Vacuolar ATPase subunitVLowNoXX
vps-11R06F6.2Vacuolar protein sorting factorVLowNoX
zen-4M03D4.1Plus-end kinesin-like motor proteinCUnd.Yes
zyg-9F22B5.7Microtubule-association proteinCLowDiffuseYes
AT-rich areaaLowNoX
B0361.10SNARE synaptobrevin/VAMPGLowNoX
C01G10.14Major sperm protein (MSP) domainLowNoX
C06A5.1Putative integrator complex subunitSLow in late embryoYesXX
C34D1.2DM DNA-binding domainLowNoX
C35A5.4Major sperm protein (MSP) domainLowNoX
C41G7.3TSPO homolog. Putative cholesterol transporterHigh in P cellsLarge granules in early embryoNoXX
C42D4.13UnknownLowNoX
F01G4.3DEAD/DEAH box helicase domainLowDiffuseNoX
F15E11.5aUnknownLowNoX
F22B5.10TMCO1 homologCUnd. in someNoXX
F29G6.1Serine protease inhibitor domainLowDiffuseNoX
F55C5.7Protein kinase, MIT, PX domainsLowNoX
F59D6.7Calcium-binding pH regulatorLowNoX
F59E10.3ζ-Subunit of coatomer (COPI)GLowDiffuseLowNoX
F59E12.11aUnknownDiffuseNoXX
K06H7.1Serine/threonine protein kinaseLowDiffuseYes
K12H4.3rRNA maturation of 60S ribosomeLowNoX
T03G6.3PhosphodiesteraseLowNoX
T04A8.7Glucosidase acid β-orthologsLow or und.NoXX
T06E6.1Possible 60S ribosomal Nop7 factorLowNoX
T12D8.6Calmodulin containingLow or und.Yes
T19C3.6UnknownLowNoX
Y47D3A.29DNA polymerase primaseLowDiffuse in P cellsYes
ZC477.4UnknownLowNoX
  • a Targeted gene originally misannotated in Ahringer RNAi Library. Correct sequenced target is shown.

  • b Gene obtained more than once in the screen.

  • c Gene class letter code (see Table 2).

  • d Und., undetectable.