TABLE 5

Properties of W148L and A179E in hflB or ΔC-term backgrounds

Strain no. NCMGenotypeDoubling time (min)MA uptakea (% wild type)
4236Wild type45100
4199ΔC-term9533
4275fs1587.0
4590amtBΔ210
4433W148L, fs47130
4798W148L, fs, hflB62340
4628W148L, ΔC-term63260
4418A179E, fs5657
4797A179E, fs, hflB62120
4820Al79E, ΔC-term60120
4683A179E796.0
4799A179E, hflB64100
  • a Data were taken from the 5-min points in Figure 4. Values for the null strain were subtracted. Uptake in wild type was 250 pmol/(ml × OD600). We have previously set our limit of reliable detection to ≤7% of wild type.